BPPH6:RDRP

Species (Taxon ID)	Pseudomonas phage phi6 (Bacteriophage phi-6). (10879)
Gene Name(s)	P2
Protein Name(s)	RNA-directed RNA polymerase Protein P2
External Links
UniProt	P11124
EMBL	M17461
PIR	B29885
RefSeq	NP_620346.1
PDB	1HHS 1HHT 1HI0 1HI1 1HI8 1UVI 1UVJ 1UVK 1UVL 1UVM 1UVN 1WAC 2JL9 2JLF 2JLG 4A8F 4A8K 4A8M 4A8O 4A8Q 4A8S 4A8W 4A8Y 4B02
PDBsum	1HHS 1HHT 1HI0 1HI1 1HI8 1UVI 1UVJ 1UVK 1UVL 1UVM 1UVN 1WAC 2JL9 2JLF 2JLG 4A8F 4A8K 4A8M 4A8O 4A8Q 4A8S 4A8W 4A8Y 4B02
ProteinModelPortal	P11124
SMR	P11124
GeneID	956436
EvolutionaryTrace	P11124
Proteomes	UP000002610
GO	GO:0019012 GO:0046872 GO:0000166 GO:0003723 GO:0050265 GO:0003968 GO:0006351 GO:0001172 GO:0019079
InterPro	IPR016406 IPR001205 IPR007096
Pfam	PF00680
PIRSF	PIRSF004131
PROSITE	PS50522

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0003968	RNA-directed RNA polymerase activity	PMID:18940872^[1]	ECO:0000315			F	Figure 3B shows that the wild type protein synthesizes RNA from ssRNA template at a faster rate than a mutant RdRp.	complete CACAO 5975
	GO:0003968	RNA-directed RNA polymerase activity	PMID:17237359^[2]	ECO:0000314			F	Figure 2A shows dsRNA generated by ϕ6 RdRP from ssRNA.	complete CACAO 5976
	GO:0003968	RNA-directed RNA polymerase activity	PMID:11602748^[3]	ECO:0000314			F	Figure 3B shows purified phi6 RdRp reacts with ssRNA to make dsRNA.	complete CACAO 5977
	GO:0003968	RNA-directed RNA polymerase activity	PMID:10619851^[4]	ECO:0000314			F	Figure 2 "Recombinant P2 catalyzes RNA-dependent RNA synthesis in vitro."	complete CACAO 5980
	GO:0003968	RNA-directed RNA polymerase activity	PMID:18614640^[5]	ECO:0000315			F	Figure 1A lanes 1 and 2 show that purified wild type P2 catalyzes the polymerization of ssRNA into dsRNA, whereas a mutant P2 does not.	complete CACAO 5981
	GO:0050265	RNA uridylyltransferase activity	PMID:18614640^[5]	ECO:0000314			F	Figure 2 shows that purified P2, when in the presence of ssRNA and excess UTP "the P2 polymerase typically added one or two extra nucleotides to a given acceptor ssRNA oligonucleotide"	complete CACAO 5982
	GO:0003968	RNA-directed RNA polymerase activity	PMID:11877396^[6]	ECO:0000314			F	Figure 2B shows that wild type phi6 RdRp was able to utilize the ssRNA substrate and form dsRNA product.	complete CACAO 5986
	GO:0039696	RNA-templated viral transcription	PMID:11080173^[7]	ECO:0000314			P	Figure 2b shows dsRNA formation proportional to P2 polymerase concentration(lanes 3-6). Also, no dsRNA was found when P2 polymerase was substituted with bovine serum albumin (lane 2).	complete CACAO 12926
enables	GO:0050265	RNA uridylyltransferase activity	PMID:18614640^[5]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	PMID:18940872^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	PMID:18614640^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	PMID:17237359^[2]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	PMID:11602748^[3]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	PMID:10619851^[4]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0003968	P	Seeded From UniProt	complete
enables	GO:0003723	RNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001205	F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001205 InterPro:IPR007096	F	Seeded From UniProt	complete
involved_in	GO:0006351	transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001205	P	Seeded From UniProt	complete
involved_in	GO:0019079	viral genome replication	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR007096	P	Seeded From UniProt	complete
involved_in	GO:0039694	viral RNA genome replication	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR016406	P	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:2.7.7.48	F	Seeded From UniProt	complete
enables	GO:0016779	nucleotidyltransferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0548	F	Seeded From UniProt	complete
part_of	GO:0019012	virion	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0946 UniProtKB-SubCell:SL-0274	C	Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F	Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0696	F	Seeded From UniProt	complete
enables	GO:0046872	metal ion binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0479	F	Seeded From UniProt	complete
enables	GO:0016740	transferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0808	F	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 Poranen, MM et al. (2008) Structural explanation for the role of Mn2+ in the activity of phi6 RNA-dependent RNA polymerase. Nucleic Acids Res. 36 6633-44 PubMed GONUTS page
↑ ^2.0 ^2.1 Aalto, AP et al. (2007) Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage phi6 RNA-dependent RNA polymerase. RNA 13 422-9 PubMed GONUTS page
↑ ^3.0 ^3.1 Yang, H et al. (2001) Comparison of polymerase subunits from double-stranded RNA bacteriophages. J. Virol. 75 11088-95 PubMed GONUTS page
↑ ^4.0 ^4.1 Makeyev, EV & Bamford, DH (2000) Replicase activity of purified recombinant protein P2 of double-stranded RNA bacteriophage phi6. EMBO J. 19 124-33 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 ^5.3 Poranen, MM et al. (2008) Nontemplated terminal nucleotidyltransferase activity of double-stranded RNA bacteriophage phi6 RNA-dependent RNA polymerase. J. Virol. 82 9254-64 PubMed GONUTS page
↑ Laurila, MR et al. (2002) Bacteriophage phi 6 RNA-dependent RNA polymerase: molecular details of initiating nucleic acid synthesis without primer. J. Biol. Chem. 277 17117-24 PubMed GONUTS page
↑ Makeyev, EV & Bamford, DH (2000) The polymerase subunit of a dsRNA virus plays a central role in the regulation of viral RNA metabolism. EMBO J. 19 6275-84 PubMed GONUTS page

[PMID:18940872-1] 1.0 ^1.1 Poranen, MM et al. (2008) Structural explanation for the role of Mn2+ in the activity of phi6 RNA-dependent RNA polymerase. Nucleic Acids Res. 36 6633-44 PubMed GONUTS page

[PMID:17237359-2] 2.0 ^2.1 Aalto, AP et al. (2007) Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage phi6 RNA-dependent RNA polymerase. RNA 13 422-9 PubMed GONUTS page

[PMID:11602748-3] 3.0 ^3.1 Yang, H et al. (2001) Comparison of polymerase subunits from double-stranded RNA bacteriophages. J. Virol. 75 11088-95 PubMed GONUTS page

[PMID:10619851-4] 4.0 ^4.1 Makeyev, EV & Bamford, DH (2000) Replicase activity of purified recombinant protein P2 of double-stranded RNA bacteriophage phi6. EMBO J. 19 124-33 PubMed GONUTS page

[PMID:18614640-5] 5.0 ^5.1 ^5.2 ^5.3 Poranen, MM et al. (2008) Nontemplated terminal nucleotidyltransferase activity of double-stranded RNA bacteriophage phi6 RNA-dependent RNA polymerase. J. Virol. 82 9254-64 PubMed GONUTS page

[PMID:11877396-6] Laurila, MR et al. (2002) Bacteriophage phi 6 RNA-dependent RNA polymerase: molecular details of initiating nucleic acid synthesis without primer. J. Biol. Chem. 277 17117-24 PubMed GONUTS page

[PMID:11080173-7] Makeyev, EV & Bamford, DH (2000) The polymerase subunit of a dsRNA virus plays a central role in the regulation of viral RNA metabolism. EMBO J. 19 6275-84 PubMed GONUTS page

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BPPH6:RDRP

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