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BPPH6:RDRP
Contents
Species (Taxon ID) | Pseudomonas phage phi6 (Bacteriophage phi-6). (10879) | |
Gene Name(s) | P2 | |
Protein Name(s) | RNA-directed RNA polymerase
Protein P2 | |
External Links | ||
UniProt | P11124 | |
EMBL | M17461 | |
PIR | B29885 | |
RefSeq | NP_620346.1 | |
PDB | 1HHS 1HHT 1HI0 1HI1 1HI8 1UVI 1UVJ 1UVK 1UVL 1UVM 1UVN 1WAC 2JL9 2JLF 2JLG 4A8F 4A8K 4A8M 4A8O 4A8Q 4A8S 4A8W 4A8Y 4B02 | |
PDBsum | 1HHS 1HHT 1HI0 1HI1 1HI8 1UVI 1UVJ 1UVK 1UVL 1UVM 1UVN 1WAC 2JL9 2JLF 2JLG 4A8F 4A8K 4A8M 4A8O 4A8Q 4A8S 4A8W 4A8Y 4B02 | |
ProteinModelPortal | P11124 | |
SMR | P11124 | |
GeneID | 956436 | |
EvolutionaryTrace | P11124 | |
Proteomes | UP000002610 | |
GO | GO:0019012 GO:0046872 GO:0000166 GO:0003723 GO:0050265 GO:0003968 GO:0006351 GO:0001172 GO:0019079 | |
InterPro | IPR016406 IPR001205 IPR007096 | |
Pfam | PF00680 | |
PIRSF | PIRSF004131 | |
PROSITE | PS50522 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0003968 |
RNA-directed RNA polymerase activity |
ECO:0000315 |
F |
Figure 3B shows that the wild type protein synthesizes RNA from ssRNA template at a faster rate than a mutant RdRp. |
complete | |||||
GO:0003968 |
RNA-directed RNA polymerase activity |
ECO:0000314 |
F |
Figure 2A shows dsRNA generated by ϕ6 RdRP from ssRNA. |
complete | |||||
GO:0003968 |
RNA-directed RNA polymerase activity |
ECO:0000314 |
F |
Figure 3B shows purified phi6 RdRp reacts with ssRNA to make dsRNA. |
complete | |||||
GO:0003968 |
RNA-directed RNA polymerase activity |
ECO:0000314 |
F |
Figure 2 "Recombinant P2 catalyzes RNA-dependent RNA synthesis in vitro." |
complete | |||||
GO:0003968 |
RNA-directed RNA polymerase activity |
ECO:0000315 |
F |
Figure 1A lanes 1 and 2 show that purified wild type P2 catalyzes the polymerization of ssRNA into dsRNA, whereas a mutant P2 does not. |
complete | |||||
GO:0050265 |
RNA uridylyltransferase activity |
ECO:0000314 |
F |
Figure 2 shows that purified P2, when in the presence of ssRNA and excess UTP "the P2 polymerase typically added one or two extra nucleotides to a given acceptor ssRNA oligonucleotide" |
complete | |||||
GO:0003968 |
RNA-directed RNA polymerase activity |
ECO:0000314 |
F |
Figure 2B shows that wild type phi6 RdRp was able to utilize the ssRNA substrate and form dsRNA product. |
complete | |||||
GO:0039696 |
RNA-templated viral transcription |
ECO:0000314 |
P |
Figure 2b shows dsRNA formation proportional to P2 polymerase concentration(lanes 3-6). Also, no dsRNA was found when P2 polymerase was substituted with bovine serum albumin (lane 2). |
complete | |||||
enables |
GO:0050265 |
RNA uridylyltransferase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
enables |
GO:0003723 |
RNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0006351 |
transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0019079 |
viral genome replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0039694 |
viral RNA genome replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0019012 |
virion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Poranen, MM et al. (2008) Structural explanation for the role of Mn2+ in the activity of phi6 RNA-dependent RNA polymerase. Nucleic Acids Res. 36 6633-44 PubMed GONUTS page
- ↑ 2.0 2.1 Aalto, AP et al. (2007) Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage phi6 RNA-dependent RNA polymerase. RNA 13 422-9 PubMed GONUTS page
- ↑ 3.0 3.1 Yang, H et al. (2001) Comparison of polymerase subunits from double-stranded RNA bacteriophages. J. Virol. 75 11088-95 PubMed GONUTS page
- ↑ 4.0 4.1 Makeyev, EV & Bamford, DH (2000) Replicase activity of purified recombinant protein P2 of double-stranded RNA bacteriophage phi6. EMBO J. 19 124-33 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 5.3 Poranen, MM et al. (2008) Nontemplated terminal nucleotidyltransferase activity of double-stranded RNA bacteriophage phi6 RNA-dependent RNA polymerase. J. Virol. 82 9254-64 PubMed GONUTS page
- ↑ Laurila, MR et al. (2002) Bacteriophage phi 6 RNA-dependent RNA polymerase: molecular details of initiating nucleic acid synthesis without primer. J. Biol. Chem. 277 17117-24 PubMed GONUTS page
- ↑ Makeyev, EV & Bamford, DH (2000) The polymerase subunit of a dsRNA virus plays a central role in the regulation of viral RNA metabolism. EMBO J. 19 6275-84 PubMed GONUTS page