PMID:11566998

Citation	Campbell, JW and Cronan, JE Jr (2001) Escherichia coli FadR positively regulates transcription of the fabB fatty acid biosynthetic gene. J. Bacteriol. 183:5982-90
Abstract	In Escherichia coli expression of the genes of fatty acid degradation (fad) is negatively regulated at the transcriptional level by FadR protein. In contrast the unsaturated fatty acid biosynthetic gene, fabA, is positively regulated by FadR. We report that fabB, a second unsaturated fatty acid biosynthetic gene, is also positively regulated by FadR. Genomic array studies that compared global transcriptional differences between wild-type and fadR-null mutant strains, as well as in cultures of each strain grown in the presence of exogenous oleic acid, indicated that expression of fabB was regulated in a manner very similar to that of fabA expression. A series of genetic and biochemical tests confirmed these observations. Strains containing both fabB and fadR mutant alleles were constructed and shown to exhibit synthetic lethal phenotypes, similar to those observed in fabA fadR mutants. A fadR strain was hypersensitive to cerulenin, an antibiotic that at low concentrations specifically targets the FabB protein. A transcriptional fusion of chloramphenicol acetyltransferase (CAT) to the fabB promoter produces lower levels of CAT protein in a strain lacking functional FadR. The ability of a putative FadR binding site within the fabB promoter to form a complex with purified FadR protein was determined by a gel mobility shift assay. These experiments demonstrate that expression of fabB is positively regulated by FadR.
Links	PubMed PMC99677 Online version:10.1128/JB.183.20.5982-5990.2001
Keywords	3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/antagonists & inhibitors; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics; Bacterial Proteins/metabolism; Base Sequence; Cerulenin/pharmacology; Enzyme Inhibitors; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Fatty Acids, Unsaturated/biosynthesis; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Genes, Lethal; Isoenzymes/antagonists & inhibitors; Isoenzymes/genetics; Molecular Sequence Data; Mutation; Repressor Proteins/metabolism; Transcription, Genetic
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:FADR		GO:0019217 : regulation of fatty acid metabolic process	ECO:0000316:	UniProtKB:P0A6Q3 UniProtKB:P0A953	P		Table 3: fabA mutants compromised in catalytic efficiency are synthetically lethal with null fadR mutations. fabB mutants compromised in catalytic efficiency are also synthetically lethal with null fadR mutations.	complete CACAO 5269
ECOLI:FADR		GO:0045723: positive regulation of fatty acid biosynthetic process	ECO:0000315:		P		Results under heading "Decreased expression of fabB in fadR strains": The promoter of the fatty acid biosynthetic gene fabB was transcriptionally fused with a promoterless CAT gene. CAT confers resistance to chloramphenicol. A strain with the transcriptional fusion and a fadR null mutation tolerated significantly lower levels of chloramphenicol than the strain with the transcriptional fusion and a functional fadR gene.	complete CACAO 5417
ECOLI:FADR	involved_in	GO:0019217: regulation of fatty acid metabolic process	ECO:0000316: genetic interaction evidence used in manual assertion		P		Seeded From UniProt	Missing: with/from
ECOLI:FADR	involved_in	GO:0045723: positive regulation of fatty acid biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ECOLI:FADR	involved_in	GO:0019217: regulation of fatty acid metabolic process	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:P0A6Q3 UniProtKB:P0A953	P		Seeded From UniProt	complete

References

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