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PMID:11562488
| Citation |
Voets, T, Moser, T, Lund, PE, Chow, RH, Geppert, M, Südhof, TC and Neher, E (2001) Intracellular calcium dependence of large dense-core vesicle exocytosis in the absence of synaptotagmin I. Proc. Natl. Acad. Sci. U.S.A. 98:11680-5 |
|---|---|
| Abstract |
Synaptotagmin I is a synaptic vesicle-associated protein essential for synchronous neurotransmission. We investigated its impact on the intracellular Ca(2+)-dependence of large dense-core vesicle (LDCV) exocytosis by combining Ca(2+)-uncaging and membrane capacitance measurements in adrenal slices from mouse synaptotagmin I null mutants. Synaptotagmin I-deficient chromaffin cells displayed prolonged exocytic delays and slow, yet Ca(2+)-dependent fusion rates, resulting in strongly reduced LDCV release in response to short depolarizations. Vesicle recruitment, the shape of individual amperometric events, and endocytosis appeared unaffected. These findings demonstrate that synaptotagmin I is required for rapid, highly Ca(2+)-sensitive LDCV exocytosis and indicate that it regulates the equilibrium between a slowly releasable and a readily releasable state of the fusion machinery. Alternatively, synaptotagmin I could function as calcium sensor for the readily releasable pool, leading to the destabilization of the pool in its absence. |
| Links |
PubMed PMC58789 Online version:10.1073/pnas.201398798 |
| Keywords |
Adrenal Glands/physiology; Animals; Animals, Newborn; Calcium/metabolism; Calcium-Binding Proteins/deficiency; Calcium-Binding Proteins/metabolism; Chromaffin Cells/physiology; Exocytosis/physiology; Intracellular Fluid/physiology; Membrane Glycoproteins/deficiency; Membrane Glycoproteins/metabolism; Mice; Mice, Knockout; Nerve Tissue Proteins/deficiency; Nerve Tissue Proteins/metabolism; Synaptotagmin I; Synaptotagmins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0017158: regulation of calcium ion-dependent exocytosis |
ECO:0000315: |
P |
Fig 2 & Fig 4. Figure 2D. shows a decrease in the exocytotic burst amplitude of SYT1 mutants. |
complete | ||||
|
involved_in |
GO:0017158: regulation of calcium ion-dependent exocytosis |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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