PMID:11546797

Citation	Xi, X, Han, J and Zhang, JZ (2001) Stimulation of glucose transport by AMP-activated protein kinase via activation of p38 mitogen-activated protein kinase. J. Biol. Chem. 276:41029-34
Abstract	Activation of AMP-activated protein kinase (AMPK) has been recently demonstrated to be associated with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR)-stimulated glucose transport mediated by both GLUT1 and GLUT4 transporters. However, signaling events upstream and downstream of AMPK are unknown. Here we report that 1) p38 mitogen-activated protein kinase (MAPK) and mitogen-activated protein kinase kinase 3 (MKK3) were activated by AICAR in Clone 9 cells, which express only the GLUT1 transporters, and 2) activation of p38 was required for AICAR-stimulated glucose transport since treatment of the cells with p38 inhibitor SB203580 or overexpression of dominant negative p38 mutant inhibited glucose transport. Moreover, we found that overexpression of the constitutively active form of AMPK mutant also resulted in a significant activation of p38, and inhibition of p38 activity by SB203580 did not affect AICAR-stimulated activation of AMPK. These findings demonstrate that AICAR-stimulated activation of p38 is indeed mediated by AMPK, and the p38 MAPK cascade is downstream of AMPK in the signaling pathway of AICAR-stimulated glucose transport in Clone 9 cells.
Links	PubMed Online version:10.1074/jbc.M102824200
Keywords	AMP-Activated Protein Kinases; Amino Acid Sequence; Aminoimidazole Carboxamide/analogs & derivatives; Aminoimidazole Carboxamide/pharmacology; Animals; Base Sequence; Biological Transport; DNA Primers; Enzyme Activation; Enzyme Inhibitors/pharmacology; Glucose/metabolism; Imidazoles/pharmacology; Mitogen-Activated Protein Kinases/genetics; Mitogen-Activated Protein Kinases/metabolism; Molecular Sequence Data; Multienzyme Complexes/antagonists & inhibitors; Multienzyme Complexes/metabolism; Protein-Serine-Threonine Kinases/antagonists & inhibitors; Protein-Serine-Threonine Kinases/metabolism; Pyridines/pharmacology; Rats; Ribonucleotides/pharmacology; p38 Mitogen-Activated Protein Kinases
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:AAPK1	involved_in	GO:0000187: activation of MAPK activity	ECO:0000303: author statement without traceable support used in manual assertion		P		Seeded From UniProt	complete

References

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