PMID:11512661

Citation	Nishizawa, Y, Usukura, J, Singh, DP, Chylack, LT Jr and Shinohara, T (2001) Spatial and temporal dynamics of two alternatively spliced regulatory factors, lens epithelium-derived growth factor (ledgf/p75) and p52, in the nucleus. Cell Tissue Res. 305:107-14
Abstract	Regulatory factors, lens epithelium-derived growth factor (LEDGF)/p75 and p52, are generated from a single LEDGF gene by alternative splicing. They have identical amino acid residues between positions 1-325, but 205 and 8 of the remaining residues are different in LEDGF and p52, respectively. LEDGF promotes growth and survival of many cell types. It has an antiapoptotic function and is a weak general transcriptional co-activator. p52 is a transcriptional activator and an essential splicing factor. We investigated the spatial and temporal dynamics of LEDGF/p75 and p52, each being tagged with a fluorescent protein, during the cell cycles of CHO-K1, MCDK, and NRK cells in culture. Both LEDGF/p75 and p52 were localized predominantly in the nucleus. LEDGF/p75 was distributed diffusely in the nucleoplasm in the G1-phase and attached to chromatin heterogeneously during the G2 and M-phases of cells. In contrast, p52 was localized in the nuclear periphery during the G1-phase and formed a speckle pattern at the S-phase. It formed a cylindrical pattern around the chromosomes during the M-phases of cells. LEDGF and p52 on sister chromatids migrated into daughter cells. Thus, LEDGF/p75 and p52 are localized in distinct nuclear compartments where they can activate transcription or splicing of pre-mRNAs.
Links	PubMed
Keywords	Alternative Splicing/physiology; Animals; Antibodies; CHO Cells; Cell Compartmentation/physiology; Cell Nucleus/genetics; Chromatids/physiology; Cricetinae; G1 Phase/physiology; G2 Phase/physiology; Green Fluorescent Proteins; Growth Substances/genetics; Growth Substances/immunology; Growth Substances/metabolism; Immunohistochemistry; Indicators and Reagents/metabolism; Intercellular Signaling Peptides and Proteins; Kidney/cytology; Luminescent Proteins/genetics; Mitosis/physiology; Protein Binding/physiology; RNA Precursors/physiology; Rats
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:PSIP1	part_of	GO:0034399: nuclear periphery	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
HUMAN:PSIP1	NOT|part_of	GO:0005730: nucleolus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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