PMID:11452083

Citation	Hutvágner, G, McLachlan, J, Pasquinelli, AE, Bálint, E, Tuschl, T and Zamore, PD (2001) A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293:834-8
Abstract	The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.
Links	PubMed Online version:10.1126/science.1062961
Keywords	Animals; Blotting, Northern; Drosophila melanogaster; Endoribonucleases/genetics; Endoribonucleases/metabolism; Gene Expression Regulation, Developmental; HeLa Cells; Humans; Nucleic Acid Conformation; Protein Structure, Tertiary; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional; RNA, Double-Stranded/metabolism; RNA, Helminth/chemistry; RNA, Helminth/genetics; RNA, Helminth/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Ribonuclease III; Transcription, Genetic; Transfection
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:DICER	enables	GO:0004525: ribonuclease III activity	ECO:0000269: experimental evidence used in manual assertion		F		Seeded From UniProt	complete

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