PMID:11316797

Citation	Cohen, CJ, Gaetz, J, Ohman, T and Bergelson, JM (2001) Multiple regions within the coxsackievirus and adenovirus receptor cytoplasmic domain are required for basolateral sorting. J. Biol. Chem. 276:25392-8
Abstract	The coxsackievirus and adenovirus receptor (CAR) mediates attachment and infection by coxsackie B viruses and many adenoviruses. In human airway epithelia, as well as in transfected Madin-Darby canine kidney cells, CAR is expressed exclusively on the basolateral surface. Variants of CAR that lack the cytoplasmic domain or are attached to the cell membrane by a glycosylphosphatidylinositol anchor are expressed on both the apical and basolateral surfaces. We have examined the localization of CAR variants with progressive truncations of the cytoplasmic domain, as well as with mutations that ablate a potential PDZ (PSD95/dlg/ZO-1) interaction motif and a putative tyrosine-based sorting signal. In addition, we have examined the targeting of two murine CAR isoforms, with different C-terminal sequences. The results suggest that multiple regions within the CAR cytoplasmic domain contain information that is necessary for basolateral targeting.
Links	PubMed Online version:10.1074/jbc.M009531200
Keywords	Adenoviridae; Amino Acid Sequence; Animals; Basement Membrane/virology; Cell Line; Cell Polarity; Cytoplasm/virology; Dogs; Enterovirus; Fluorescent Antibody Technique; Mice; Molecular Sequence Data; Mutation; Receptors, Virus/metabolism; Structure-Activity Relationship; Tyrosine/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:CXAR	part_of	GO:0016323: basolateral plasma membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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