GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:11222604
| Citation |
Hazan, R, Sat, B, Reches, M and Engelberg-Kulka, H (2001) Postsegregational killing mediated by the P1 phage "addiction module" phd-doc requires the Escherichia coli programmed cell death system mazEF. J. Bacteriol. 183:2046-50 |
|---|---|
| Abstract |
"Addiction modules" consist of two genes; the product of the second is long lived and toxic, while the product of the first is short lived and antagonizes the lethal action of the toxin. The extrachromosomal addiction module phd-doc, located on the P1 prophage, is responsible for the postsegregational killing effect (death of plasmid-free cells). The Escherichia coli chromosomal addiction module analogue, mazEF, is responsible for the induction of programmed cell death. Here we show that the postsegregational killing mediated by the P1 phd-doc module depends on the presence of the E. coli mazEF system. In addition, we demonstrate that under conditions of postsegregational killing, mediated by phd-doc, protein synthesis of E. coli is inhibited. Based on our findings, we suggest the existence of a coupling between the phd-doc and mazEF systems. |
| Links |
PubMed PMC95101 Online version:10.1128/JB.183.6.2046-2050.2001 |
| Keywords |
Antitoxins/genetics; Antitoxins/metabolism; Apoptosis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Toxins/genetics; Bacteriophage P1/genetics; Bacteriophage P1/physiology; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli/virology; Plasmids/genetics; Viral Proteins/genetics; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044696: killing by virus of host cell by post-segregational killing |
ECO:0000315: |
P |
Fig (1b) shows that growth the E.coli MC4100 cells is disrupted by introduction of the phd-doc gene into the pGBts plasmid. |
complete | ||||
| GO:0044074: negative regulation by symbiont of host translation |
ECO:0000315: |
P |
Fig (3) shows that E.coli cell with the phd-doc plasmid and produced less methionine than cells without the phd-doc system. |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
