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PMID:11222603
| Citation |
Sat, B, Hazan, R, Fisher, T, Khaner, H, Glaser, G and Engelberg-Kulka, H (2001) Programmed cell death in Escherichia coli: some antibiotics can trigger mazEF lethality. J. Bacteriol. 183:2041-5 |
|---|---|
| Abstract |
The discovery of toxin-antitoxin gene pairs (also called addiction modules) on extrachromosomal elements of Escherichia coli, and particularly the discovery of homologous modules on the bacterial chromosome, suggest that a potential for programmed cell death may be inherent in bacterial cultures. We have reported on the E. coli mazEF system, a regulatable addiction module located on the bacterial chromosome. MazF is a stable toxin and MazE is a labile antitoxin. Here we show that cell death mediated by the E. coli mazEF module can be triggered by several antibiotics (rifampicin, chloramphenicol, and spectinomycin) that are general inhibitors of transcription and/or translation. These antibiotics inhibit the continuous expression of the labile antitoxin MazE, and as a result, the stable toxin MazF causes cell death. Our results have implications for the possible mode(s) of action of this group of antibiotics. |
| Links |
PubMed PMC95100 Online version:10.1128/JB.183.6.2041-2045.2001 |
| Keywords |
Anti-Bacterial Agents/pharmacology; Antitoxins/genetics; Antitoxins/metabolism; Apoptosis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Toxins/genetics; Bacterial Toxins/metabolism; Culture Media; Escherichia coli/drug effects; Escherichia coli/growth & development; Gene Expression Regulation, Bacterial; Protein Biosynthesis/drug effects; Transcription, Genetic/drug effects |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0043068: positive regulation of programmed cell death |
ECO:0000315: |
P |
Fig (1a) shows that more E.coli cells with a disrupted clpP gene survived treatment with the antibiotics rifampin, chloramphenicol, and spectinomycin than wild type cells. |
complete | ||||
| GO:0043068: positive regulation of programmed cell death |
ECO:0000315: |
P |
Fig (1a,b,c) shows that more E.coli cells with a disrupted mazEF gene survived treatment with the antibiotics rifampin, chloramphenicol, and spectinomycin (all of which inhibit transcription) than wild type cells. |
complete | ||||
Notes
See also
References
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