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PMID:11034318
| Citation |
Glaser, W and Skern, T (2000) Extremely efficient cleavage of eIF4G by picornaviral proteinases L and 2A in vitro. FEBS Lett. 480:151-5 |
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| Abstract |
Certain picornaviruses encode proteinases which cleave the translation initiation factor eIF4G, a member of the eIF4F complex which recruits mRNA to the 40S ribosomal subunit during initiation of protein synthesis in eukaryotes. We have compared the efficiency of eIF4G cleavage in rabbit reticulocyte lysates during translation of mRNAs encoding the foot-and-mouth disease virus leader proteinase (Lpro) or the human rhinovirus 2Apro. Under standard translation conditions, Lpro cleaved 50% of eIF4G within 4 min after initiation of protein synthesis, whereas 2Apro required 15 min. At these times, the molar ratios of proteinase to eIF4G were 1:130 for Lpro and 1:12 for 2Apro, indicating a much more efficient in vitro cleavage than previously observed. The molar ratios are similar to those observed during viral infection in vivo. |
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| Keywords |
Animals; Cysteine Endopeptidases/biosynthesis; Cysteine Endopeptidases/genetics; Cysteine Endopeptidases/metabolism; Endopeptidases/biosynthesis; Endopeptidases/genetics; Endopeptidases/metabolism; Eukaryotic Initiation Factor-4G; Humans; Peptide Initiation Factors/metabolism; Rabbits; Viral Proteins |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0039611: suppression by virus of host translation initiation factor activity |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
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