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PMID:10858262

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Citation

Lai, XH, Arencibia, I, Johansson, A, Wai, SN, Oscarsson, J, Kalfas, S, Sundqvist, KG, Mizunoe, Y, Sjöstedt, A and Uhlin, BE (2000) Cytocidal and apoptotic effects of the ClyA protein from Escherichia coli on primary and cultured monocytes and macrophages. Infect. Immun. 68:4363-7

Abstract

Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates a hemolytic phenotype. We show here that highly purified ClyA and ClyA-expressing E. coli were cytotoxic and apoptogenic to fresh as well as cultured human and murine monocytes/macrophages.

Links

PubMed PMC101772

Keywords

Animals; Apoptosis/drug effects; Cell Death/drug effects; Cell Line; DNA Fragmentation/drug effects; Escherichia coli/genetics; Escherichia coli/pathogenicity; Escherichia coli Proteins; Hemolysin Proteins/genetics; Hemolysin Proteins/isolation & purification; Hemolysin Proteins/toxicity; Humans; In Vitro Techniques; Macrophages/drug effects; Macrophages/pathology; Mice; Monocytes/drug effects; Monocytes/pathology; U937 Cells

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:HLYE

acts_upstream_of_or_within

GO:0001897: cytolysis by symbiont of host cells

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:HLYE

acts_upstream_of_or_within

GO:0044532: modulation of apoptotic process in other organism

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:HLYE

GO:0009405: pathogenesis

ECO:0000315:

P

Figure 1: Cytotoxicity as manifested by LDH release from murine macrophages J774. Figure 2: The viability of freshly isolated human peripheral monocytes showed a dose-related decline after treatment with ClyA for 20 h

complete
CACAO 10704

ECOLI:HLYE

involved_in

GO:0051715: cytolysis in other organism

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:HLYE

involved_in

GO:0009405: pathogenesis

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:HLYE

involved_in

GO:0044532: modulation of apoptotic process in other organism

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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