PMID:10758502

Citation	Liu, Z, Hossain, GS, Islas-Osuna, MA, Mitchell, DL and Mount, DW (2000) Repair of UV damage in plants by nucleotide excision repair: Arabidopsis UVH1 DNA repair gene is a homolog of Saccharomyces cerevisiae Rad1. Plant J. 21:519-28
Abstract	To analyze plant mechanisms for resistance to UV radiation, mutants of Arabidopsis that are hypersensitive to UV radiation (designated uvh and uvr) have been isolated. UVR2 and UVR3 products were previously identified as photolyases that remove UV-induced pyrimidine dimers in the presence of visible light. Plants also remove dimers in the absence of light by an as yet unidentified dark repair mechanism and uvh1 mutants are defective in this mechanism. The UVH1 locus was mapped to chromosome 5 and the position of the UVH1 gene was further delineated by Agrobacterium-mediated transformation of the uvh1-1 mutant with cosmids from this location. Cosmid NC23 complemented the UV hypersensitive phenotype and restored dimer removal in the uvh1-1 mutant. The cosmid encodes a protein similar to the S. cerevisiae RAD1 and human XPF products, components of an endonuclease that excises dimers by nucleotide excision repair (NER). The uvh1-1 mutation creates a G to A transition in intron 5 of this gene, resulting in a new 3' splice site and introducing an in-frame termination codon. These results provide evidence that the Arabidopsis UVH1/AtRAD1 product is a subunit of a repair endonuclease. The previous discovery in Lilium longiflorum of a homolog of human ERCC1 protein that comprises the second subunit of the repair endonuclease provides additional evidence for the existence of the repair endonuclease in plants. The UVH1 gene is strongly expressed in flower tissue and also in other tissues, suggesting that the repair endonuclease is widely utilized for repair of DNA damage in plant tissues.
Links	PubMed
Keywords	Amino Acid Sequence; Arabidopsis/genetics; Chromosome Mapping; Chromosomes; DNA Damage; DNA Repair; DNA Repair Enzymes; DNA-Binding Proteins; Endonucleases/genetics; Gene Expression Regulation, Plant; Humans; Molecular Sequence Data; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Ultraviolet Rays
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:XPF		GO:0000720: pyrimidine dimer repair by nucleotide-excision repair	ECO:0000315:		P		Figure 2D shows the recovery of the repair of (6-4) photoproduct in uvh1 plants transformed with UVH1/AtRAD1. Recovery is seen in both WT and transformed uvh1. Organism: Arabidopsis thaliana	complete CACAO 12971
ARATH:XPF		GO:0006289: nucleotide-excision repair	ECO:0000247:	UniProtKB:Q92889	P		Figure 4 shows sequence alignment to XPF human (and other homologous DNA proteins for nucleotide excision repair) to UVH1. Organism: Arabidopsis thaliana	complete CACAO 12992

References

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