GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Schwartz, PT, Perez-Villamil, B, Rivera, A, Moratalla, R and Vallejo, M (2000) Pancreatic homeodomain transcription factor IDX1/IPF1 expressed in developing brain regulates somatostatin gene transcription in embryonic neural cells. J. Biol. Chem. 275:19106-14


Hox-like homeodomain proteins play a critical role during embryonic development by regulating the transcription of genes that are important for the generation of specific organs or cell types. The homeodomain transcription factor IDX1/IPF1, the expression of which was thought until recently to be restricted to the pancreas and foregut, is required for pancreas development and for the expression of genes controlling glucose homeostasis. We report that IDX1/IPF1 is also expressed in embryonic rat brain at a time coincident with active neurogenesis. Electrophoretic mobility shift assays with nuclear extracts of embryonic brains indicated that IDX1/IPF1 binds to two somatostatin promoter elements, SMS-UE-B and the recently discovered SMS-TAAT3. The requirement of these elements for IDX1/IPF1 transactivation of the somatostatin gene in neural cells was confirmed in transfection studies using embryonic cerebral cortex-derived RC2.E10 cells. Immunohistochemical staining of rat embryos showed IDX1/IPF1-positive cells located near the ventricular surface in germinative areas of the developing central nervous system. Cellular colocalization of IDX1/IPF1 and somatostatin was found in several areas of the developing brain, including cortex, ganglionic eminence, hypothalamus, and inferior colliculus. These results support the notion that IDX1/IPF1 regulates gene expression during development of the central nervous system independent of its role on pancreas development and function.


PubMed Online version:10.1074/jbc.M000655200


Animals; Base Sequence; Brain/embryology; Brain/metabolism; DNA Primers; Gene Expression Regulation/physiology; Genes, Homeobox; Homeodomain Proteins; Immunohistochemistry; Pancreas/metabolism; Rats; Regulatory Sequences, Nucleic Acid; Reverse Transcriptase Polymerase Chain Reaction; Somatostatin/genetics; Trans-Activators/chemistry; Trans-Activators/physiology; Transcription, Genetic



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0007417: central nervous system development

ECO:0000270: expression pattern evidence used in manual assertion


Seeded From UniProt


See also


See Help:References for how to manage references in GONUTS.