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PMID:10644350
| Citation |
Bui, M, Wills, EG, Helenius, A and Whittaker, GR (2000) Role of the influenza virus M1 protein in nuclear export of viral ribonucleoproteins. J. Virol. 74:1781-6 |
|---|---|
| Abstract |
The protein kinase inhibitor H7 blocks influenza virus replication, inhibits production of the matrix protein (M1), and leads to a retention of the viral ribonucleoproteins (vRNPs) in the nucleus at late times of infection (K. Martin and A. Helenius, Cell 67:117-130, 1991). We show here that production of assembled vRNPs occurs normally in H7-treated cells, and we have used H7 as a biochemical tool to trap vRNPs in the nucleus. When H7 was removed from the cells, vRNP export was specifically induced in a CHO cell line stably expressing recombinant M1. Similarly, fusion of cells expressing recombinant M1 from a Semliki Forest virus vector allowed nuclear export of vRNPs. However, export was not rescued when H7 was present in the cells, implying an additional role for phosphorylation in this process. The viral NS2 protein was undetectable in these systems. We conclude that influenza virus M1 is required to induce vRNP nuclear export but that cellular phosphorylation is an additional factor. |
| Links |
PubMed PMC111655 Online version:10.1128/jvi.74.4.1781-1786.2000 |
| Keywords |
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology; Animals; Biological Transport; CHO Cells; Cattle; Cell Line; Cell Nucleus/metabolism; Cricetinae; Enzyme Inhibitors/pharmacology; HeLa Cells; Humans; Influenza A virus/metabolism; Mice; Ribonucleoproteins/biosynthesis; Ribonucleoproteins/drug effects; Ribonucleoproteins/isolation & purification; Ribonucleoproteins/metabolism; Viral Matrix Proteins/metabolism; Viral Matrix Proteins/physiology; Viral Proteins/biosynthesis; Viral Proteins/drug effects; Viral Proteins/isolation & purification; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0042025: host cell nucleus |
ECO:0005804: immunofluorescence evidence used in manual assertion |
C |
Fig. 4 Shows M1 is required for NP export from the host nucleus. Organism: Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) UniProtKB:P05777 (M1_I33A0) UniProt submitted name: Matrix protein 1 |
complete | ||||
| GO:0071426: ribonucleoprotein complex export from nucleus |
ECO:0005804: immunofluorescence evidence used in manual assertion |
P |
Fig. 4 shows that infected CHO cells stably expressing M1 are able to export NP to the cytoplasm while infected CHO cells not expressing M1 cannot. Organism: Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) UniProtKB: P05777 (M1_I33A0) UniProt submitted name: Matrix protein 1 |
complete | ||||
Notes
See also
References
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