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PMID:10559162

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Citation

Kim, JS, Chang, JH, Chung, SI and Yum, JS (1999) Molecular cloning and characterization of the Helicobacter pylori fliD gene, an essential factor in flagellar structure and motility. J. Bacteriol. 181:6969-76

Abstract

Helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. Flagellar motility is regarded as a major factor in the colonizing ability of H. pylori. The functional roles of flagellar structural proteins other than FlaA, FlaB, and FlgE are not well understood. The fliD operon of H. pylori consists of flaG, fliD, and fliS genes, in the order stated, under the control of a sigma(28)-dependent promoter. In an effort to elucidate the function of the FliD protein, a hook-associated protein 2 homologue, in flagellar morphogenesis and motility, the fliD gene (2,058 bp) was cloned and isogenic mutants were constructed by disruption of the fliD gene with a kanamycin resistance cassette and electroporation-mediated allelic-exchange mutagenesis. In the fliD mutant, morphologically abnormal flagellar appendages in which very little filament elongation was apparent were observed. The fliD mutant strain was completely nonmotile, indicating that these abnormal flagella were functionally defective. Furthermore, the isogenic fliD mutant of H. pylori SS1, a mouse-adapted strain, was not able to colonize the gastric mucosae of host mice. These results suggest that H. pylori FliD is an essential element in the assembly of the functional flagella that are required for colonization of the gastric mucosa.

Links

PubMed PMC94171

Keywords

Animals; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cloning, Molecular; Duodenal Ulcer/microbiology; Electroporation; Flagella/chemistry; Flagella/physiology; Flagellin/genetics; Flagellin/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Helicobacter Infections/microbiology; Helicobacter pylori/genetics; Helicobacter pylori/physiology; Helicobacter pylori/ultrastructure; Humans; Mice; Microscopy, Electron; Molecular Sequence Data; Restriction Mapping; Sequence Analysis, DNA

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HELPY:FLID

GO:0071978: bacterial-type flagellar swarming motility

ECO:0000315:

P

Figure 5: Motility testing of H. pylori wild-type and isogenic fliD mutant strains revealed that the wild-type strain formed colonies with the large diffuse spreading halo typical of motile bacteria, while the fliD mutant strain showed no apparent motility (produced small and and sharply delineated colonies).

complete
CACAO 6249

HELPY:FLID

GO:0009288: bacterial-type flagellum

ECO:0000315:

C

Figure 3: In wild-type cells, full length sheathed flagella are observed. Within the wild-type flagellar sheath, filaments are elongated to the tips of the flagella and terminal bulbs are observed. In the fliD mutant, truncated flagella are observed and terminal bulbs are not apparent. Filament elongation is not observed in the fliD mutant.

complete
CACAO 6252

HELPY:FLID

GO:0007155: cell adhesion

ECO:0000315:

P

Table 2: No H. pylori cells could be recovered from the gastric tissue of the mice inoculated orally with the fliD mutant strain. The researchers recovered 2.23 x 10^5 cells per gram of gastric tissue from the mice inoculated orally with wild-type H. pylori.

complete
CACAO 6256

HELPY:FLID

part_of

GO:0009288: bacterial-type flagellum

ECO:0000315: mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

HELPY:FLID

involved_in

GO:0071978: bacterial-type flagellum-dependent swarming motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


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Backtrace:

#0 /usr/local/github/wiki-extensions/wiki-extensions-hulab/AnnotationLinks/AnnotationLinks.php(70): AnnotationLinks::getQuickGOannotations(string, array)
#1 /var/www/html/go/wiki/includes/parser/Parser.php(3888): AnnotationLinks::renderAnnotations(NULL, array, Parser, PPFrame_DOM)
#2 /var/www/html/go/wiki/includes/parser/Preprocessor_DOM.php(1362): Parser->extensionSubstitution(array, PPFrame_DOM)
#3 /var/www/html/go/wiki/includes/parser/Parser.php(2942): PPFrame_DOM->expand(DOMElement, integer)
#4 /var/www/html/go/wiki/includes/parser/Parser.php(1293): Parser->replaceVariables(string)
#5 /var/www/html/go/wiki/includes/parser/Parser.php(443): Parser->internalParse(string)
#6 /var/www/html/go/wiki/includes/content/WikitextContent.php(323): Parser->parse(string, Title, ParserOptions, boolean, boolean, integer)
#7 /var/www/html/go/wiki/includes/content/AbstractContent.php(516): WikitextContent->fillParserOutput(Title, integer, ParserOptions, boolean, ParserOutput)
#8 /var/www/html/go/wiki/includes/content/ContentHandler.php(1324): AbstractContent->getParserOutput(Title, integer, ParserOptions)
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#10 /usr/local/github/wiki-extensions/wiki-extensions-hulab/CirrusSearch/includes/Updater.php(407): CirrusSearch\Updater::buildDocument(CirrusSearch, WikiPage, CirrusSearch\Connection, integer, integer, integer)
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#12 /usr/local/github/wiki-extensions/wiki-extensions-hulab/CirrusSearch/includes/Updater.php(83): CirrusSearch\Updater->updatePages(array, integer)
#13 /usr/local/github/wiki-extensions/wiki-extensions-hulab/CirrusSearch/includes/Job/LinksUpdate.php(52): CirrusSearch\Updater->updateFromTitle(Title)
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#15 /var/www/html/go/wiki/includes/jobqueue/JobRunner.php(296): CirrusSearch\Job\Job->run()
#16 /var/www/html/go/wiki/includes/jobqueue/JobRunner.php(193): JobRunner->executeJob(CirrusSearch\Job\LinksUpdate, Wikimedia\Rdbms\LBFactorySimple, BufferingStatsdDataFactory, integer)
#17 /var/www/html/go/wiki/includes/MediaWiki.php(1002): JobRunner->run(array)
#18 /var/www/html/go/wiki/includes/MediaWiki.php(988): MediaWiki->triggerSyncJobs(integer, MediaWiki\Logger\LegacyLogger)
#19 /var/www/html/go/wiki/includes/MediaWiki.php(912): MediaWiki->triggerJobs()
#20 /var/www/html/go/wiki/includes/MediaWiki.php(727): MediaWiki->restInPeace(string, boolean)
#21 /var/www/html/go/wiki/includes/MediaWiki.php(750): MediaWiki->{closure}()
#22 /var/www/html/go/wiki/includes/MediaWiki.php(557): MediaWiki->doPostOutputShutdown(string)
#23 /var/www/html/go/wiki/index.php(42): MediaWiki->run()
#24 {main}

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