PMID:10559162

Citation	Kim, JS, Chang, JH, Chung, SI and Yum, JS (1999) Molecular cloning and characterization of the Helicobacter pylori fliD gene, an essential factor in flagellar structure and motility. J. Bacteriol. 181:6969-76
Abstract	Helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. Flagellar motility is regarded as a major factor in the colonizing ability of H. pylori. The functional roles of flagellar structural proteins other than FlaA, FlaB, and FlgE are not well understood. The fliD operon of H. pylori consists of flaG, fliD, and fliS genes, in the order stated, under the control of a sigma(28)-dependent promoter. In an effort to elucidate the function of the FliD protein, a hook-associated protein 2 homologue, in flagellar morphogenesis and motility, the fliD gene (2,058 bp) was cloned and isogenic mutants were constructed by disruption of the fliD gene with a kanamycin resistance cassette and electroporation-mediated allelic-exchange mutagenesis. In the fliD mutant, morphologically abnormal flagellar appendages in which very little filament elongation was apparent were observed. The fliD mutant strain was completely nonmotile, indicating that these abnormal flagella were functionally defective. Furthermore, the isogenic fliD mutant of H. pylori SS1, a mouse-adapted strain, was not able to colonize the gastric mucosae of host mice. These results suggest that H. pylori FliD is an essential element in the assembly of the functional flagella that are required for colonization of the gastric mucosa.
Links	PubMed PMC94171
Keywords	Animals; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cloning, Molecular; Duodenal Ulcer/microbiology; Electroporation; Flagella/chemistry; Flagella/physiology; Flagellin/genetics; Flagellin/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Helicobacter Infections/microbiology; Helicobacter pylori/genetics; Helicobacter pylori/physiology; Helicobacter pylori/ultrastructure; Humans; Mice; Microscopy, Electron; Molecular Sequence Data; Restriction Mapping; Sequence Analysis, DNA
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HELPY:FLID		GO:0071978: bacterial-type flagellar swarming motility	ECO:0000315:		P		Figure 5: Motility testing of H. pylori wild-type and isogenic fliD mutant strains revealed that the wild-type strain formed colonies with the large diffuse spreading halo typical of motile bacteria, while the fliD mutant strain showed no apparent motility (produced small and and sharply delineated colonies).	complete CACAO 6249
HELPY:FLID		GO:0009288: bacterial-type flagellum	ECO:0000315:		C		Figure 3: In wild-type cells, full length sheathed flagella are observed. Within the wild-type flagellar sheath, filaments are elongated to the tips of the flagella and terminal bulbs are observed. In the fliD mutant, truncated flagella are observed and terminal bulbs are not apparent. Filament elongation is not observed in the fliD mutant.	complete CACAO 6252
HELPY:FLID		GO:0007155: cell adhesion	ECO:0000315:		P		Table 2: No H. pylori cells could be recovered from the gastric tissue of the mice inoculated orally with the fliD mutant strain. The researchers recovered 2.23 x 10^5 cells per gram of gastric tissue from the mice inoculated orally with wild-type H. pylori.	complete CACAO 6256
HELPY:FLID	part_of	GO:0009288: bacterial-type flagellum	ECO:0000315: mutant phenotype evidence used in manual assertion		C		Seeded From UniProt	complete
HELPY:FLID	involved_in	GO:0071978: bacterial-type flagellum-dependent swarming motility	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.