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PMID:10534118
| Citation |
Masso-Welch, PA, Black, JD, Erikson, J and Repasky, EA (1999) Polarized expression of immunoglobulin, spectrin, and protein kinase C beta II occurs in B cells from normal BALB/c, autoimmune lpr, and anti-ssDNA transgenic, tolerant mice. J. Leukoc. Biol. 66:617-24 |
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| Abstract |
The rapid redistribution of B cell surface immunoglobulin to a cap upon cross-linking treatment is a well-described phenomenon, the physiological significance of which is unknown. We describe the observation that splenic B cells from unimmunized normal, autoimmune, and tolerant mice express naturally occurring capped immunoglobulin in the absence of exogenous stimulation. The percentage of capped B cells increases to 20% of B cells by age 16 weeks in the progressive autoimmune lpr mouse. Transgenic, tolerant mice expressing lpr-derived genes for ssDNA-binding antibody also demonstrate a large percentage (35-75%) of immunoglobulin-capped splenic B cells. In these capped B cells, protein kinase C beta II, the cytoskeletal proteins spectrin and ankyrin, and the lipophilic probe diI are enriched beneath the site of the immunoglobulin cap. These data suggest that polarization of surface receptors, signaling molecules, anionic phospholipid domains, and cytoskeletal proteins may be an important part of the B cell immune response in vivo. |
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| Keywords |
Animals; Autoimmunity; B-Lymphocytes/immunology; B-Lymphocytes/metabolism; Carbocyanines; Cell Membrane; Cell Polarity; DNA, Single-Stranded/immunology; Fluorescent Antibody Technique; Fluorescent Dyes; Gene Expression; Immune Tolerance/immunology; Immunoglobulin Heavy Chains/biosynthesis; Immunoglobulin Heavy Chains/genetics; Immunoglobulin Light Chains/biosynthesis; Immunoglobulin Light Chains/genetics; Immunoglobulins/biosynthesis; Immunoglobulins/genetics; Isoenzymes/biosynthesis; Mice; Mice, Inbred BALB C; Mice, Inbred MRL lpr; Mice, Transgenic; Protein Kinase C/biosynthesis; Protein Kinase C beta; Spectrin/biosynthesis; Staining and Labeling/methods |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008091: spectrin |
ECO:0000314: |
C |
Fig. 5. PKC beta II is concentrated within caps of spectrin in B cells. B cells from BALB/c (a), lpr (b), or anti-ssDNA transgenic mice were stained using rabbit anti-spectrin antibody detected with donkey anti-rabbit fluorescein,followed by biotinylated mouse anti-PKC beta-II antibody detected with streptavidin Texas red. Arrows indicate PKC beta-II enriched in the submembranous region of B cells with capped spectrin. The image shown here is the summation of confocal images collected at 2-μm intervals. Left side of image, spectrin staining. Right side of image, PKC beta-II staining of the same field of cells. PKC Beta II is an isoform of the the protein KPCB |
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Notes
See also
References
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