PMID:10398532

Citation	Jasoni, C, Hendrickson, A and Roelink, H (1999) Analysis of chicken Wnt-13 expression demonstrates coincidence with cell division in the developing eye and is consistent with a role in induction. Dev. Dyn. 215:215-24
Abstract	We used a degenerate polymerase chain reaction (PCR) strategy to search for Wnt RNA in developing ocular tissues. We isolated a Macaca monkey Wnt-13 PCR fragment, orthologous to the human and murine Wnt-13 and Xenopus Wnt-2b, and a chick Wnt13 cDNA. Wnt-13 is a member of the Wnt-1 class of transforming Wnt molecules. In situ RNA hybridization revealed a dynamic Wnt-13 expression pattern in numerous developing tissues. Within the eye, Wnt-13 is expressed in the proliferative epithelium of the lens and both pigmented and non-pigmented layers of the ciliary margin. In vitro BrdU incorporation studies coupled with in situ hybridization showed that cWnt-13 expression domains in the lens were coincident with cell division. In addition to the eye, cWnt-13 was expressed in head ectoderm, prospective forelimb mesenchyme, lung bud, pharyngeal arches, the brain, as well as the otic vesicle. Our data are consistent with previous observations linking transforming Wnts with cell division and implicate a cascade of events involving cWnt-13 first in dorsoventral patterning and later in cell proliferation regulation associated with lens development. Dev Dyn 1999;215:215-224.
Links	PubMed Online version:<215::AID-AJA4>3.0.CO;2-W 10.1002/(SICI)1097-0177(199907)215:3<215::AID-AJA4>3.0.CO;2-W
Keywords	Amino Acid Sequence; Animals; Cell Division; Chick Embryo; Chickens/genetics; Embryonic Induction/physiology; Eye Proteins/biosynthesis; Eye Proteins/genetics; Gene Expression Regulation, Developmental; Glycoproteins/biosynthesis; Glycoproteins/genetics; Humans; In Situ Hybridization; Intercellular Signaling Peptides and Proteins; Lens, Crystalline/embryology; Macaca nemestrina; Mice; Molecular Sequence Data; Morphogenesis/genetics; Organ Specificity; Pigment Epithelium of Eye/metabolism; Polymerase Chain Reaction; RNA, Messenger/biosynthesis; Sequence Alignment; Sequence Homology, Amino Acid; Wnt Proteins; Xenopus laevis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
CHICK:WNT2B	involved_in	GO:0008283: cell population proliferation	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete
CHICK:Q9PUI3	involved_in	GO:0008283: cell population proliferation	ECO:0000270: expression pattern evidence used in manual assertion		P		Seeded From UniProt	complete

References

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