PMID:10364345

Citation	Zou, P, Isegawa, Y, Nakano, K, Haque, M, Horiguchi, Y and Yamanishi, K (1999) Human herpesvirus 6 open reading frame U83 encodes a functional chemokine. J. Virol. 73:5926-33
Abstract	Some viruses including herpesviruses have undergone evolution to benefit viral infection and propagation by pirating and modifying host genes such as chemokine genes. Human herpesvirus 6 (HHV-6), acutely or persistently infects mononuclear cells in vitro. DNA sequence analysis of HHV-6 has revealed that the putative protein encoded by an open reading frame (ORF) of the U83 gene in HHV-6 variant B resembled a human chemokine. We have cloned the U83 gene and analyzed the biological function of this gene. The U83 gene contained an ORF encoding a 113-amino-acid peptide, starting at the first methionine and containing a possible signal peptide and the typical cysteine residues characteristic of the chemokines. Reverse transcription-PCR analysis of mRNA and immunofluorescent-antibody testing of infected cells both indicated that the encoded protein was a late protein. The ORF U83 gene fused to the Fc gene was expressed as a fusion protein in COS-7 cells by transfection, and the fusion protein was purified from the supernatant of transfected cells to test its biological function. The purified protein was capable of inducing transient calcium mobilization in THP-1 cells and of chemotactically activating THP-1 cells. These findings suggested that the U83 protein might play an important role in HHV-6 propagation in vivo by activating and trafficking mononuclear cells to sites of viral replication, thus aiding the development of superbly efficient virus production mechanisms.
Links	PubMed PMC112654
Keywords	Amino Acid Sequence; Animals; Base Sequence; COS Cells; Calcium/metabolism; Cell Line; Chemokines/genetics; Chemokines/metabolism; Chemotaxis/physiology; Cloning, Molecular; DNA, Viral; Gene Expression; Genes, Viral; Herpesvirus 6, Human/genetics; Humans; Immunoglobulin Fc Fragments/genetics; Molecular Sequence Data; Open Reading Frames; Recombinant Fusion Proteins/genetics; Reverse Transcriptase Polymerase Chain Reaction; Viral Proteins/genetics; Viral Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HHV6Z:CCU83	enables	GO:0008009: chemokine activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
HHV6Z:CCU83		GO:0008009: chemokine activity	ECO:0000314:		F		Fig 7. shows that cells were induced to migrate by the protein.	complete CACAO 7884

References

See Help:References for how to manage references in GONUTS.