GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

ECOLI:ERA

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) era (synonyms: rbaA, sdgE)
Protein Name(s) GTPase Era

ERA GTP-binding protein Era

External Links
UniProt P06616
EMBL M14658
D64044
U36841
U00096
AP009048
X02673
PIR S44713
RefSeq NP_417061.1
YP_490794.1
PDB 1EGA
3IEU
PDBsum 1EGA
3IEU
ProteinModelPortal P06616
SMR P06616
DIP DIP-9521N
IntAct P06616
MINT MINT-1248282
STRING 511145.b2566
PhosSite P010424
PaxDb P06616
PRIDE P06616
EnsemblBacteria AAC75619
BAE76742
GeneID 12934226
947036
KEGG ecj:Y75_p2519
eco:b2566
PATRIC 32120531
EchoBASE EB0266
EcoGene EG10270
eggNOG COG1159
HOGENOM HOG000245597
InParanoid P06616
KO K03595
OMA KVAKDWQ
OrthoDB EOG68DD1Q
PhylomeDB P06616
BioCyc EcoCyc:EG10270-MONOMER
ECOL316407:JW2550-MONOMER
EvolutionaryTrace P06616
PRO PR:P06616
Proteomes UP000000318
UP000000625
Genevestigator P06616
GO GO:0005737
GO:0005829
GO:0005886
GO:0005525
GO:0003924
GO:0043024
GO:0070181
GO:0006184
GO:0006468
GO:0042274
Gene3D 3.30.300.20
3.40.50.300
HAMAP MF_00367
InterPro IPR030388
IPR005662
IPR006073
IPR015946
IPR004044
IPR009019
IPR027417
IPR005225
Pfam PF07650
PF01926
SUPFAM SSF52540
SSF54814
TIGRFAMs TIGR00436
TIGR00231
PROSITE PS51713
PS50823

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0003924

GTPase activity

PMID:2105934[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Figure 8A. shows that soluble forms of Era, when coupled with GTP, yield both GTP and GDP. This is evidence of GTPase activity.

complete
CACAO 3826

GO:0005886

plasma membrane

PMID:8282709[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Figure 5A. shows results of an Era Binding Assay. Era binds to subfractions of the membrane. This is also evidence of Era's presence in the membrane.

complete
CACAO 3827

part_of

GO:0005886

plasma membrane

PMID:8282709[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0003924

GTPase activity

PMID:2105934[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0070181

small ribosomal subunit rRNA binding

PMID:10527840[3]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043024

ribosomal small subunit binding

PMID:10527840[3]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0042274

ribosomal small subunit biogenesis

PMID:12753192[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006468

protein phosphorylation

PMID:8919456[5]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

PMID:8282709[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0005525

GTP binding

PMID:3097637[6]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0003924

GTPase activity

PMID:10094501[7]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043024

ribosomal small subunit binding

PMID:21873635[8]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10270
PANTHER:PTN000182760
UniProtKB:O75616

F

Seeded From UniProt

complete

enables

GO:0019843

rRNA binding

PMID:21873635[8]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000182760
UniProtKB:O75616

F

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:21873635[8]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10270
PANTHER:PTN000182823
UniProtKB:P9WNK9

C

Seeded From UniProt

complete

involved_in

GO:0000028

ribosomal small subunit assembly

PMID:21873635[8]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000182760
UniProtKB:O75616

P

Seeded From UniProt

complete

involved_in

GO:0046777

protein autophosphorylation

PMID:8919456[5]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0042274

ribosomal small subunit biogenesis

PMID:16825789[9]

ECO:0000316

genetic interaction evidence used in manual assertion

UniProtKB:P0A7G2

P

Seeded From UniProt

complete

involved_in

GO:0042274

ribosomal small subunit biogenesis

PMID:20188109[10]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0031234

extrinsic component of cytoplasmic side of plasma membrane

PMID:1925028[11]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:17272300[12]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0003723

RNA binding

PMID:10510227[13]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0003723

RNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004044
InterPro:IPR005662
InterPro:IPR009019

F

Seeded From UniProt

complete

enables

GO:0005525

GTP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR005225
InterPro:IPR005662
InterPro:IPR006073
InterPro:IPR030388

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084139

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084139

C

Seeded From UniProt

complete

enables

GO:0070181

small ribosomal subunit rRNA binding

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084139

F

Seeded From UniProt

complete

enables

GO:0005525

GTP binding

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084139

F

Seeded From UniProt

complete

involved_in

GO:0042274

ribosomal small subunit biogenesis

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084139

P

Seeded From UniProt

complete

enables

GO:0003924

GTPase activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000084139

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

PMID:8282709[2]

ECO:0000304

author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0997
UniProtKB-KW:KW-1003
UniProtKB-SubCell:SL-0037

C

Seeded From UniProt

complete

involved_in

GO:0042254

ribosome biogenesis

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0690

P

Seeded From UniProt

complete

enables

GO:0005525

GTP binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0342

F

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

enables

GO:0000166

nucleotide binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

enables

GO:0019843

rRNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0699

F

Seeded From UniProt

complete

enables

GO:0003723

RNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0694

F

Seeded From UniProt

complete

edit table

Notes

References

See Help:References for how to manage references in GONUTS.

  1. ↑ 1.0 1.1 Chen, SM et al. (1990) Expression and characterization of RNase III and Era proteins. Products of the rnc operon of Escherichia coli. J. Biol. Chem. 265 2888-95 PubMed GONUTS page
  2. ↑ 2.0 2.1 2.2 2.3 Lin, YP et al. (1994) GTPase-dependent signaling in bacteria: characterization of a membrane-binding site for era in Escherichia coli. J. Bacteriol. 176 44-9 PubMed GONUTS page
  3. ↑ 3.0 3.1 Sayed, A et al. (1999) Era, an essential Escherichia coli small G-protein, binds to the 30S ribosomal subunit. Biochem. Biophys. Res. Commun. 264 51-4 PubMed GONUTS page
  4. ↑ Inoue, K et al. (2003) Suppression of defective ribosome assembly in a rbfA deletion mutant by overexpression of Era, an essential GTPase in Escherichia coli. Mol. Microbiol. 48 1005-16 PubMed GONUTS page
  5. ↑ 5.0 5.1 Shimamoto, T & Inouye, M (1996) Mutational analysis of Era, an essential GTP-binding protein of Escherichia coli. FEMS Microbiol. Lett. 136 57-62 PubMed GONUTS page
  6. ↑ Ahnn, J et al. (1986) A GTP-binding protein of Escherichia coli has homology to yeast RAS proteins. Proc. Natl. Acad. Sci. U.S.A. 83 8849-53 PubMed GONUTS page
  7. ↑ Chen, X et al. (1999) Purification, characterization and crystallization of ERA, an essential GTPase from Escherichia coli. FEBS Lett. 445 425-30 PubMed GONUTS page
  8. ↑ 8.0 8.1 8.2 8.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  9. ↑ Inoue, K et al. (2006) Era and RbfA have overlapping function in ribosome biogenesis in Escherichia coli. J. Mol. Microbiol. Biotechnol. 11 41-52 PubMed GONUTS page
  10. ↑ Bunner, AE et al. (2010) The effect of ribosome assembly cofactors on in vitro 30S subunit reconstitution. J. Mol. Biol. 398 1-7 PubMed GONUTS page
  11. ↑ Gollop, N & March, PE () Localization of the membrane binding sites of Era in Escherichia coli. Res. Microbiol. 142 301-7 PubMed GONUTS page
  12. ↑ Watt, RM et al. (2007) Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes. Nucleic Acids Res. 35 e37 PubMed GONUTS page
  13. ↑ Johnstone, BH et al. (1999) The widely conserved Era G-protein contains an RNA-binding domain required for Era function in vivo. Mol. Microbiol. 33 1118-31 PubMed GONUTS page
Retrieved from "https://gowiki.tamu.edu/wiki/index.php?title=ECOLI:ERA&oldid=5960816"