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PMID:10527840

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Citation

Sayed, A, Matsuyama, S and Inouye, M (1999) Era, an essential Escherichia coli small G-protein, binds to the 30S ribosomal subunit. Biochem. Biophys. Res. Commun. 264:51-4

Abstract

Era is an essential G-protein in Escherichia coli identified originally as a homologue protein to Ras (E. coli Ras-like protein). It binds to GTP/GDP and contains a low intrinsic GTPase activity. Its function remains elusive, although it has been suggested that Era is associated with the cytoplasmic membrane, cell division, energy metabolism, and cell-cycle check point. Recently, a cold-sensitive phenotype was found to be suppressed by the overexpression of 16S rRNA methyltransferase, suggesting Era association with the ribosome. Here we demonstrate that Era specifically binds to 16S rRNA and the 30S ribosomal subunit. Both GTP and GDP, but not GMP, inhibit Era binding to ribosomal component. Involvement of Era in protein synthesis is suggested by the fact that Era depletion results in the translation defect both in vitro and in vivo.

Links

PubMed Online version:10.1006/bbrc.1999.1471

Keywords

5'-Guanylic Acid/metabolism; Bacterial Proteins/metabolism; Cell Division; Escherichia coli/metabolism; Escherichia coli Proteins; GTP-Binding Proteins/metabolism; Guanosine Triphosphate/metabolism; Peptide Biosynthesis; Protein Binding; RNA, Ribosomal, 16S/metabolism; RNA-Binding Proteins; Ribosomes/metabolism; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:ERA

enables

GO:0043024: ribosomal small subunit binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:ERA

enables

GO:0070181: small ribosomal subunit rRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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