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DROME:SPT5H

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Species (Taxon ID) Drosophila melanogaster (Fruit fly). (7227)
Gene Name(s) Spt5
Protein Name(s) Transcription elongation factor SPT5

DRB sensitivity-inducing factor large subunit DSIF large subunit dSpt5

External Links
UniProt Q9V460
EMBL AF222864
AE013599
AY069140
BT001668
BT023840
RefSeq NP_652610.1
UniGene Dm.2263
ProteinModelPortal Q9V460
SMR Q9V460
BioGrid 72729
DIP DIP-59538N
IntAct Q9V460
MINT MINT-322311
PaxDb Q9V460
PRIDE Q9V460
EnsemblMetazoa FBtr0086531
GeneID 53442
KEGG dme:Dmel_CG7626
UCSC CG7626-RA
CTD 53442
FlyBase FBgn0040273
eggNOG COG5164
GeneTree ENSGT00440000037640
InParanoid Q9V460
KO K15172
OMA WEDGNPA
OrthoDB EOG7Z3F3Q
PhylomeDB Q9V460
Reactome REACT_209653
REACT_217308
REACT_217571
REACT_221895
REACT_226178
GenomeRNAi 53442
NextBio 841174
PRO PR:Q9V460
Proteomes UP000000803
Bgee Q9V460
GO GO:0032044
GO:0005700
GO:0005705
GO:0005703
GO:0008023
GO:0046982
GO:0007549
GO:0007095
GO:0007052
GO:0032785
GO:0000122
GO:0032786
GO:0045944
GO:0006368
InterPro IPR005824
IPR006645
IPR024945
IPR022581
IPR017071
IPR005100
IPR008991
Pfam PF00467
PF03439
PF11942
PIRSF PIRSF036945
SMART SM01104
SM00739
SM00738
SUPFAM SSF50104

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

Contributes to

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:12782658[1]

ECO:0000314

P

Fig. 1 DSIF (formed from DROME:SPT4H and DROME:SPT5H) is shown to cause promoter proximal pausing by monitoring the reactivity of the transcribed DNA to permanganate. Immunodepletion of DSIF inhibits promoter proximal pausing, and restoring DSIF restores promoter proximal pausing.

complete

GO:0008148

negative transcription elongation factor activity

PMID:12782658[1]

ECO:0000314

F

Fig. 1 DSIF (formed from DROME:SPT4H and DROME:SPT5H) is shown to cause promoter proximal pausing by monitoring the reactivity of the transcribed DNA to permanganate. Immunodepletion of DSIF inhibits promoter proximal pausing, and restoring DSIF restores promoter proximal pausing.

complete

GO:0005634

nucleus

PMID:11040216[2]

ECO:0000314

C

Fig. 2c. Immunofluorescence shows Spt5 localized in the nucleus.

complete

enables

GO:0000993

RNA polymerase II complex binding

PMID:12934007[3]

ECO:0000314

direct assay evidence used in manual assertion

F

has_direct_input:(UniProtKB:P04052)

Seeded From UniProt

complete

enables

GO:0046982

protein heterodimerization activity

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

F

Seeded From UniProt

complete

involved_in

GO:0045944

positive regulation of transcription by RNA polymerase II

PMID:15380072[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0045944

positive regulation of transcription by RNA polymerase II

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

P

Seeded From UniProt

complete

enables

GO:0044877

protein-containing complex binding

PMID:12934007[3]

ECO:0000314

direct assay evidence used in manual assertion

F

has_direct_input:(GO:0035101)

Seeded From UniProt

complete

involved_in

GO:0032786

positive regulation of DNA-templated transcription, elongation

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

P

Seeded From UniProt

complete

involved_in

GO:0032785

negative regulation of DNA-templated transcription, elongation

PMID:12782658[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0032785

negative regulation of DNA-templated transcription, elongation

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

P

Seeded From UniProt

complete

part_of

GO:0032044

DSIF complex

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

C

Seeded From UniProt

complete

part_of

GO:0008023

transcription elongation factor complex

PMID:12934007[3]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:Q9W420

C

Seeded From UniProt

complete

involved_in

GO:0006368

transcription elongation from RNA polymerase II promoter

PMID:12782658[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006368

transcription elongation from RNA polymerase II promoter

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

P

Seeded From UniProt

complete

part_of

GO:0005705

polytene chromosome interband

PMID:12782658[1]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005705

polytene chromosome interband

PMID:11040216[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005703

polytene chromosome puff

PMID:12934007[3]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005703

polytene chromosome puff

PMID:11040216[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005703

polytene chromosome puff

PMID:12782658[1]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005703

polytene chromosome puff

PMID:11040217[5]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

PMID:15380072[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

PMID:12782658[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

GO_REF:0000024

ECO:0000250

sequence similarity evidence used in manual assertion

UniProtKB:O00267

P

Seeded From UniProt

complete

part_of

GO:0032044

DSIF complex

PMID:21873635[6]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0040273
PANTHER:PTN000122628
PomBase:SPAC23C4.19
SGD:S000004470
UniProtKB:O00267

C

Seeded From UniProt

complete

involved_in

GO:0006368

transcription elongation from RNA polymerase II promoter

PMID:21873635[6]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0040273
PANTHER:PTN000122628
UniProtKB:O00267

P

Seeded From UniProt

complete

involved_in

GO:0006357

regulation of transcription by RNA polymerase II

PMID:21873635[6]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN000122628
ZFIN:ZDB-GENE-001207-1

P

Seeded From UniProt

complete

enables

GO:0003729

mRNA binding

PMID:21873635[6]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

FB:FBgn0040273
PANTHER:PTN000122628

F

Seeded From UniProt

complete

part_of

GO:0005700

polytene chromosome

PMID:23209435[7]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0007549

dosage compensation

PMID:23209435[7]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0003682

chromatin binding

PMID:28213523[8]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0003729

mRNA binding

PMID:28213523[8]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0032044

DSIF complex

PMID:28213523[8]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

contributes_to

GO:0000993

RNA polymerase II complex binding

PMID:28213523[8]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0000122

negative regulation of transcription by RNA polymerase II

PMID:28213523[8]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0006355

regulation of transcription, DNA-templated

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR006645
InterPro:IPR036735

P

Seeded From UniProt

complete

involved_in

GO:0006357

regulation of transcription by RNA polymerase II

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR017071
InterPro:IPR039659

P

Seeded From UniProt

complete

involved_in

GO:0032784

regulation of DNA-templated transcription, elongation

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR017071
InterPro:IPR039659

P

Seeded From UniProt

complete

part_of

GO:0005694

chromosome

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0158
UniProtKB-SubCell:SL-0468

C

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0539
UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

complete

edit table

Notes

References

See Help:References for how to manage references in GONUTS.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Wu, CH et al. (2003) NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 17 1402-14 PubMed GONUTS page
  2. ↑ 2.0 2.1 2.2 Kaplan, CD et al. (2000) Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 14 2623-34 PubMed GONUTS page
  3. ↑ 3.0 3.1 3.2 3.3 Saunders, A et al. (2003) Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo. Science 301 1094-6 PubMed GONUTS page
  4. ↑ 4.0 4.1 Jennings, BH et al. (2004) Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila. Curr. Biol. 14 1680-4 PubMed GONUTS page
  5. ↑ Andrulis, ED et al. (2000) High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 14 2635-49 PubMed GONUTS page
  6. ↑ 6.0 6.1 6.2 6.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  7. ↑ 7.0 7.1 Prabhakaran, M & Kelley, RL (2012) Mutations in the transcription elongation factor SPT5 disrupt a reporter for dosage compensation in Drosophila. PLoS Genet. 8 e1003073 PubMed GONUTS page
  8. ↑ 8.0 8.1 8.2 8.3 8.4 Qiu, Y & Gilmour, DS (2017) Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing. J. Biol. Chem. 292 5555-5570 PubMed GONUTS page
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