DROME:SPT5H

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Drosophila melanogaster (Fruit fly). (7227)
Gene Name(s) Spt5
Protein Name(s) Transcription elongation factor SPT5

DRB sensitivity-inducing factor large subunit DSIF large subunit dSpt5

External Links
UniProt Q9V460
EMBL AF222864
AE013599
AY069140
BT001668
BT023840
RefSeq NP_652610.1
UniGene Dm.2263
ProteinModelPortal Q9V460
SMR Q9V460
BioGrid 72729
DIP DIP-59538N
IntAct Q9V460
MINT MINT-322311
PaxDb Q9V460
PRIDE Q9V460
EnsemblMetazoa FBtr0086531
GeneID 53442
KEGG dme:Dmel_CG7626
UCSC CG7626-RA
CTD 53442
FlyBase FBgn0040273
eggNOG COG5164
GeneTree ENSGT00440000037640
InParanoid Q9V460
KO K15172
OMA WEDGNPA
OrthoDB EOG7Z3F3Q
PhylomeDB Q9V460
Reactome REACT_209653
REACT_217308
REACT_217571
REACT_221895
REACT_226178
GenomeRNAi 53442
NextBio 841174
PRO PR:Q9V460
Proteomes UP000000803
Bgee Q9V460
GO GO:0032044
GO:0005700
GO:0005705
GO:0005703
GO:0008023
GO:0046982
GO:0007549
GO:0007095
GO:0007052
GO:0032785
GO:0000122
GO:0032786
GO:0045944
GO:0006368
InterPro IPR005824
IPR006645
IPR024945
IPR022581
IPR017071
IPR005100
IPR008991
Pfam PF00467
PF03439
PF11942
PIRSF PIRSF036945
SMART SM01104
SM00739
SM00738
SUPFAM SSF50104

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Contributes to

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Fig. 1 DSIF (formed from DROME:SPT4H and DROME:SPT5H) is shown to cause promoter proximal pausing by monitoring the reactivity of the transcribed DNA to permanganate. Immunodepletion of DSIF inhibits promoter proximal pausing, and restoring DSIF restores promoter proximal pausing.

complete

GO:0008148

negative transcription elongation factor activity

PMID:12782658[1]

IDA: Inferred from Direct Assay

F

Fig. 1 DSIF (formed from DROME:SPT4H and DROME:SPT5H) is shown to cause promoter proximal pausing by monitoring the reactivity of the transcribed DNA to permanganate. Immunodepletion of DSIF inhibits promoter proximal pausing, and restoring DSIF restores promoter proximal pausing.

complete

GO:0005634

nucleus

PMID:11040216[2]

IDA: Inferred from Direct Assay

C

Fig. 2c. Immunofluorescence shows Spt5 localized in the nucleus.

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

Seeded From UniProt

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:15380072[3]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:15380072[3]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0005515

protein binding

PMID:14605208[4]

IPI: Inferred from Physical Interaction

UniProtKB:Q9I7J8

F

complete

GO:0005634

nucleus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

Seeded From UniProt

complete

GO:0005515

protein binding

PMID:14605208[4]

IPI: Inferred from Physical Interaction

UniProtKB:Q9U4G5

F

complete

GO:0005634

nucleus

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

complete

GO:0005694

chromosome

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0158

C

Seeded From UniProt

complete

GO:0005515

protein binding

PMID:14605208[4]

IPI: Inferred from Physical Interaction

UniProtKB:Q9VHM5

F

complete

GO:0005694

chromosome

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0468

C

Seeded From UniProt

complete

GO:0005634

nucleus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0539

C

complete

GO:0005700

polytene chromosome

PMID:23209435[5]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005634

nucleus

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0191

C

complete

GO:0005703

polytene chromosome puff

PMID:11040216[2]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005694

chromosome

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0158

C

complete

GO:0005703

polytene chromosome puff

PMID:11040217[6]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005694

chromosome

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0468

C

complete

GO:0005703

polytene chromosome puff

PMID:12782658[1]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005703

polytene chromosome puff

PMID:11040216[2]

IDA: Inferred from Direct Assay

C

complete

GO:0005703

polytene chromosome puff

PMID:12934007[7]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005703

polytene chromosome puff

PMID:11040217[6]

IDA: Inferred from Direct Assay

C

complete

GO:0005705

polytene chromosome interband

PMID:11040216[2]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005703

polytene chromosome puff

PMID:12782658[1]

IDA: Inferred from Direct Assay

C

complete

GO:0005705

polytene chromosome interband

PMID:12782658[1]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005703

polytene chromosome puff

PMID:12934007[7]

IDA: Inferred from Direct Assay

C

complete

GO:0006351

transcription, DNA-templated

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0804

P

Seeded From UniProt

complete

GO:0005705

polytene chromosome interband

PMID:11040216[2]

IDA: Inferred from Direct Assay

C

complete

GO:0006355

regulation of transcription, DNA-templated

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006645

P

Seeded From UniProt

complete

GO:0005705

polytene chromosome interband

PMID:12782658[1]

IDA: Inferred from Direct Assay

C

complete

GO:0006355

regulation of transcription, DNA-templated

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0805

P

Seeded From UniProt

complete

GO:0006351

transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

complete

GO:0006357

regulation of transcription from RNA polymerase II promoter

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017071

P

Seeded From UniProt

complete

GO:0006355

regulation of transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

complete

GO:0006368

transcription elongation from RNA polymerase II promoter

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

Seeded From UniProt

complete

GO:0006357

regulation of transcription from RNA polymerase II promoter

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017071

P

complete

GO:0006368

transcription elongation from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0006368

transcription elongation from RNA polymerase II promoter

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

complete

GO:0007052

mitotic spindle organization

PMID:17412918[8]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0006368

transcription elongation from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

complete

GO:0007095

mitotic G2 DNA damage checkpoint

PMID:21205937[9]

IGI: Inferred from Genetic Interaction

FB:FBgn0261530

P

Seeded From UniProt

complete

GO:0007052

mitotic spindle organization

PMID:17412918[8]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0007549

dosage compensation

PMID:23209435[5]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007095

mitotic cell cycle G2/M transition DNA damage checkpoint

PMID:21205937[9]

IGI: Inferred from Genetic Interaction

FB:FBgn0261530

P

complete

GO:0008023

transcription elongation factor complex

PMID:12934007[7]

IPI: Inferred from Physical Interaction

UniProtKB:Q9W420

C

Seeded From UniProt

complete

GO:0008023

transcription elongation factor complex

PMID:12934007[7]

IPI: Inferred from Physical Interaction

UniProtKB:Q9W420

C

complete

GO:0032044

DSIF complex

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

C

Seeded From UniProt

complete

GO:0032784

regulation of transcription elongation, DNA-dependent

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017071

P

complete

GO:0032784

regulation of DNA-templated transcription, elongation

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR017071

P

Seeded From UniProt

complete

GO:0032785

negative regulation of transcription elongation, DNA-dependent

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

complete

GO:0032785

negative regulation of DNA-templated transcription, elongation

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

Seeded From UniProt

complete

GO:0032785

negative regulation of transcription elongation, DNA-dependent

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

complete

GO:0032786

positive regulation of transcription elongation, DNA-dependent

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

complete

GO:0032785

negative regulation of DNA-templated transcription, elongation

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0032968

positive regulation of transcription elongation from RNA polymerase II promoter

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006645

P

complete

GO:0032786

positive regulation of DNA-templated transcription, elongation

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

Seeded From UniProt

complete

GO:0045944

positive regulation of transcription from RNA polymerase II promoter

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

complete

GO:0045944

positive regulation of transcription from RNA polymerase II promoter

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

P

Seeded From UniProt

complete

GO:0045944

positive regulation of transcription from RNA polymerase II promoter

PMID:15380072[3]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0045944

positive regulation of transcription from RNA polymerase II promoter

PMID:15380072[3]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0046982

protein heterodimerization activity

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

F

complete

GO:0046982

protein heterodimerization activity

GO_REF:0000024

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:O00267

F

Seeded From UniProt

complete

colocalizes_with

GO:0000176

nuclear exosome (RNase complex)

PMID:12490954[10]

IDA: Inferred from Direct Assay

C

complete

colocalizes_with

GO:0000176

nuclear exosome (RNase complex)

PMID:12490954[10]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

colocalizes_with

GO:0035101

FACT complex

PMID:12934007[7]

IPI: Inferred from Physical Interaction

UniProtKB:Q9W420

C

Seeded From UniProt

complete

colocalizes_with

GO:0035101

FACT complex

PMID:12934007[7]

IPI: Inferred from Physical Interaction

UniProtKB:Q9W420

C

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Wu, CH et al. (2003) NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 17 1402-14 PubMed GONUTS page
  2. 2.0 2.1 2.2 2.3 2.4 Kaplan, CD et al. (2000) Spt5 and spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 14 2623-34 PubMed GONUTS page
  3. 3.0 3.1 3.2 3.3 Jennings, BH et al. (2004) Locus-specific requirements for Spt5 in transcriptional activation and repression in Drosophila. Curr. Biol. 14 1680-4 PubMed GONUTS page
  4. 4.0 4.1 4.2 Giot, L et al. (2003) A protein interaction map of Drosophila melanogaster. Science 302 1727-36 PubMed GONUTS page
  5. 5.0 5.1 Prabhakaran, M & Kelley, RL (2012) Mutations in the transcription elongation factor SPT5 disrupt a reporter for dosage compensation in Drosophila. PLoS Genet. 8 e1003073 PubMed GONUTS page
  6. 6.0 6.1 Andrulis, ED et al. (2000) High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation. Genes Dev. 14 2635-49 PubMed GONUTS page
  7. 7.0 7.1 7.2 7.3 7.4 7.5 Saunders, A et al. (2003) Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo. Science 301 1094-6 PubMed GONUTS page
  8. 8.0 8.1 Goshima, G et al. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316 417-21 PubMed GONUTS page
  9. 9.0 9.1 Kondo, S & Perrimon, N (2011) A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. Sci Signal 4 rs1 PubMed GONUTS page
  10. 10.0 10.1 Andrulis, ED et al. () The RNA processing exosome is linked to elongating RNA polymerase II in Drosophila. Nature 420 837-41 PubMed GONUTS page