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DROME:NELFE

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Species (Taxon ID) Drosophila melanogaster (Fruit fly). (7227)
Gene Name(s) Nelf-E (synonyms: anon-66Da)
Protein Name(s) Negative elongation factor E
External Links
UniProt P92204
EMBL Y10015
Y10016
AE014296
AY071061
RefSeq NP_648241.1
UniGene Dm.20046
ProteinModelPortal P92204
SMR P92204
BioGrid 64390
IntAct P92204
MINT MINT-779659
STRING 7227.FBpp0076374
PaxDb P92204
PRIDE P92204
EnsemblMetazoa FBtr0076650
GeneID 38982
KEGG dme:Dmel_CG5994
CTD 38982
FlyBase FBgn0017430
eggNOG NOG86140
GeneTree ENSGT00630000089917
InParanoid P92204
KO K15182
OMA YNDDVYK
OrthoDB EOG7SV0W7
PhylomeDB P92204
Reactome REACT_217308
REACT_221895
REACT_226178
SignaLink P92204
GenomeRNAi 38982
NextBio 811294
PRO PR:P92204
Proteomes UP000000803
Bgee P92204
GO GO:0005694
GO:0032021
GO:0005634
GO:0008023
GO:0017053
GO:0003729
GO:0000166
GO:0003723
GO:0032785
GO:0034244
GO:0000122
GO:0007070
GO:0022008
GO:0045944
GO:0006368
Gene3D 3.30.70.330
InterPro IPR012677
IPR000504
Pfam PF00076
SMART SM00360
PROSITE PS50102

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0008148

negative transcription elongation factor activity

PMID:12782658[1]

IMP: Inferred from Mutant Phenotype

F

Fig 4. Mutants with RNAi against NELF-E show depletion in promoter proximal pausing, as detected by monitoring the reactivity of transcribed DNA to permanganate.

complete

GO:0000166

nucleotide binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012677

F

complete

GO:0003676

nucleic acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000504

F

complete

GO:0003723

RNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

complete

GO:0003723

RNA binding

PMID:12782658[1]

IDA: Inferred from Direct Assay

F

complete

GO:0005515

protein binding

PMID:14605208[2]

IPI: Inferred from Physical Interaction

UniProtKB:P17276

F

complete

GO:0005634

nucleus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0539

C

complete

GO:0005634

nucleus

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0191

C

complete

GO:0005694

chromosome

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0158

C

complete

GO:0005694

chromosome

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0468

C

complete

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

PMID:18628398[3]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0006351

transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

complete

GO:0000166

nucleotide binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012677

F

Seeded From UniProt

complete

GO:0006355

regulation of transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

complete

GO:0003676

nucleic acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000504

F

Seeded From UniProt

complete

GO:0006368

transcription elongation from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

complete

GO:0003723

RNA binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0694

F

Seeded From UniProt

complete

GO:0003723

RNA binding

PMID:12782658[1]

IDA: Inferred from Direct Assay

F

Seeded From UniProt

complete

GO:0007070

negative regulation of transcription from RNA polymerase II promoter during mitosis

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

complete

GO:0003729

mRNA binding

PMID:10908586[4]

ISS: Inferred from Sequence or Structural Similarity

F

Seeded From UniProt

Missing: with/from

GO:0008023

transcription elongation factor complex

PMID:12782658[1]

IPI: Inferred from Physical Interaction

UniProtKB:Q24134

C

complete

GO:0005515

protein binding

PMID:22579285[5]

IPI: Inferred from Physical Interaction

UniProtKB:P02828

F

Seeded From UniProt

complete

GO:0017053

transcriptional repressor complex

PMID:12782658[1]

IDA: Inferred from Direct Assay

C

complete

GO:0005634

nucleus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

Seeded From UniProt

complete

GO:0032785

negative regulation of transcription elongation, DNA-dependent

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

complete

GO:0005634

nucleus

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

complete

GO:0005634

nucleus

PMID:20534440[6]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005694

chromosome

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0158

C

Seeded From UniProt

complete

GO:0005694

chromosome

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0468

C

Seeded From UniProt

complete

GO:0006351

transcription, DNA-templated

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0804

P

Seeded From UniProt

complete

GO:0006355

regulation of transcription, DNA-templated

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0805

P

Seeded From UniProt

complete

GO:0006368

transcription elongation from RNA polymerase II promoter

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0007070

negative regulation of transcription from RNA polymerase II promoter during mitosis

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0008023

transcription elongation factor complex

PMID:12782658[1]

IPI: Inferred from Physical Interaction

UniProtKB:Q24134

C

Seeded From UniProt

complete

GO:0017053

transcriptional repressor complex

PMID:12782658[1]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0022008

neurogenesis

PMID:21549331[7]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0032021

NELF complex

PMID:20534440[6]

IPI: Inferred from Physical Interaction

FB:FBgn0010416

C

Seeded From UniProt

complete

GO:0032785

negative regulation of DNA-templated transcription, elongation

PMID:12782658[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0034244

negative regulation of transcription elongation from RNA polymerase II promoter

PMID:20534440[6]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0045944

positive regulation of transcription from RNA polymerase II promoter

PMID:18628398[3]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Wu, CH et al. (2003) NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 17 1402-14 PubMed GONUTS page
  2. Giot, L et al. (2003) A protein interaction map of Drosophila melanogaster. Science 302 1727-36 PubMed GONUTS page
  3. 3.0 3.1 Gilchrist, DA et al. (2008) NELF-mediated stalling of Pol II can enhance gene expression by blocking promoter-proximal nucleosome assembly. Genes Dev. 22 1921-33 PubMed GONUTS page
  4. Lasko, P (2000) The drosophila melanogaster genome: translation factors and RNA binding proteins. J. Cell Biol. 150 F51-6 PubMed GONUTS page
  5. Sawarkar, R et al. (2012) Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli. Cell 149 807-18 PubMed GONUTS page
  6. 6.0 6.1 6.2 Missra, A & Gilmour, DS (2010) Interactions between DSIF (DRB sensitivity inducing factor), NELF (negative elongation factor), and the Drosophila RNA polymerase II transcription elongation complex. Proc. Natl. Acad. Sci. U.S.A. 107 11301-6 PubMed GONUTS page
  7. Neumüller, RA et al. (2011) Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Cell Stem Cell 8 580-93 PubMed GONUTS page