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CAEEL:RFS1
Contents
| Species (Taxon ID) | Caenorhabditis elegans. (6239) | |
| Gene Name(s) | rfs-1 | |
| Protein Name(s) | RAD51-like protein 1 | |
| External Links | ||
| UniProt | P34348 | |
| EMBL | FO080290 | |
| PIR | S44775 | |
| RefSeq | NP_001293630.1 | |
| ProteinModelPortal | P34348 | |
| SMR | P34348 | |
| IntAct | P34348 | |
| MINT | P34348 | |
| STRING | 6239.C30A5.2 | |
| PaxDb | P34348 | |
| EnsemblMetazoa | C30A5.2 | |
| GeneID | 24104262 | |
| KEGG | cel:CELE_C30A5.2 | |
| CTD | 24104262 | |
| WormBase | C30A5.2 | |
| eggNOG | ENOG410K7J5 ENOG4110MBP | |
| HOGENOM | HOG000020888 | |
| InParanoid | P34348 | |
| OMA | IVCKRVE | |
| OrthoDB | EOG091G0QIY | |
| PhylomeDB | P34348 | |
| PRO | PR:P34348 | |
| Proteomes | UP000001940 | |
| Bgee | WBGene00004342 | |
| GO | GO:0033061 GO:0033063 GO:0005657 GO:0005524 GO:0051117 GO:0008094 GO:0003690 GO:0000400 GO:0000150 GO:0003697 GO:0000707 GO:0006312 GO:0000712 GO:0010212 GO:0042148 | |
| InterPro | IPR013632 IPR027417 IPR020588 | |
| Pfam | PF08423 | |
| SUPFAM | SSF52540 | |
| PROSITE | PS50162 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0000730 |
DNA recombinase assembly |
ECO:0000316 |
|
P |
Figure 1 depicts the cessation of the dissociation of RAD-51 from ssDNA due to the presence of protein RFS-1/RIP-1. Figure 1, Part H [(i), (ii)] shows the known mechanism of RAD-51 by demonstrating that it’s presence prohibits the binding of ScRPA-eGFP-ssDNA. The incapability of RAD-51 to be expressed due to the lack of ATP is found in Figure 1, Part H (ii). Figure 1 ultimately illustrates that in the precence of the protein RFS-1/RIP-1 and ATP; RAD-51 along with the protein RFS-1/RIP-1 is present but the ScRPA-eGFP-ssDNA are not [(Figure 1, Part H (iii)]. The same events occur when the protein RFS-1/RIP-1 is present but not ATP [(Figure 1, Part H (iv)]. |
complete | ||||
| GO:0000730 |
DNA recombinase assembly |
ECO:0000316 |
|
P |
Figure 2 provides the biochemical properties of the RFS-1/RIP-1 complex. The western blot in part A of this figure confirms the precence of the two proteins (RFS-1/RIP-1) after co-purifying the recombinant proteins from budding yeast cells. The Electrophoretic mobility shift assays (EMSA) shown in part B of the figure reveals that the RFS-1/RIP-1 complex binds in a nucleotide independent manner weakly to ssDNA but not dsDNA. Bands are observed only for ssDNA. The RFS-1/RIP-1 complex can also stimulate D loop formations with RAD51 (C) and the fact that ATP is required to do so (D). |
complete | ||||
|
part_of |
GO:0033061 |
DNA recombinase mediator complex |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051117 |
ATPase binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
WB:WBGene00004297 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000712 |
resolution of meiotic recombination intermediates |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
WB:WBGene00021905 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000712 |
resolution of meiotic recombination intermediates |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
WB:WBGene00009124 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006281 |
DNA repair |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008094 |
DNA-dependent ATPase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006310 |
DNA recombination |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051321 |
meiotic cell cycle |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006281 |
DNA repair |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006974 |
cellular response to DNA damage stimulus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Taylor, MRG et al. (2016) A Polar and Nucleotide-Dependent Mechanism of Action for RAD51 Paralogs in RAD51 Filament Remodeling. Mol. Cell 64 926-939 PubMed GONUTS page
- ↑ 2.0 2.1 Taylor, MRG et al. (2015) Rad51 Paralogs Remodel Pre-synaptic Rad51 Filaments to Stimulate Homologous Recombination. Cell 162 271-286 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 Ward, JD et al. (2010) Overlapping mechanisms promote postsynaptic RAD-51 filament disassembly during meiotic double-strand break repair. Mol. Cell 37 259-72 PubMed GONUTS page
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