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BPHK7:CAPSD
Contents
| Species (Taxon ID) | Enterobacteria phage HK97 (Bacteriophage HK97). (37554) | |
| Gene Name(s) | 5 | |
| Protein Name(s) | Major capsid protein (ECO:0000303 with PMID:17098191[1])
Gene product 5 (ECO:0000305) gp5 (ECO:0000305) Major head protein (ECO:0000303 with PMID:7723019[2]) Scaffolding domain delta | |
| External Links | ||
| UniProt | P49861 | |
| EMBL | U18319 AF069529 | |
| PIR | S54392 | |
| RefSeq | NP_037701.1 | |
| PDB | 1IF0 1OHG 2FRP 2FS3 2FSY 2FT1 2FTE 2GP1 3DDX 3E8K 3J1A 3QPR | |
| PDBsum | 1IF0 1OHG 2FRP 2FS3 2FSY 2FT1 2FTE 2GP1 3DDX 3E8K 3J1A 3QPR | |
| ProteinModelPortal | P49861 | |
| SMR | P49861 | |
| DIP | DIP-29163N | |
| GeneID | 1262530 | |
| KEGG | vg:1262530 | |
| EvolutionaryTrace | P49861 | |
| PMAP-CutDB | P49861 | |
| Proteomes | UP000002576 | |
| GO | GO:0039620 GO:0019076 | |
| InterPro | IPR006444 IPR024455 | |
| Pfam | PF05065 | |
| TIGRFAMs | TIGR01554 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0046797 |
viral procapsid maturation |
ECO:0000315 |
P |
HK97 delta domain is necessary for proper procapsid assembly. HK97 delta domain comprises the first 102 amino acids of the major capsid protein, is present during procapsid assembly, and is cleaved during capsid maturation. In Figure 2A, HK97 lacking the delta domain was found to have become insoluble. Figure 2B shows similar results, with delta-less HK97 present only in the pellet fraction (as marked by a circle). Figure 2C shows expression of a full-length HK97 protein plasmid (protease-) and delta-less HK97. Full-length HK97 is shown to properly form capsomers and proheads—signs of proper assembly— while the lack of bands for the delta-less HK97 show there is no formation of capsomers or proheads. From this, we can conclude that the delta-domain of HK97 is necessary for formation of a properly folded, soluble procapsid head. |
complete | |||||
|
part_of |
GO:0019028 |
viral capsid |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019028 |
viral capsid |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0039620 |
T=7 icosahedral viral capsid |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019012 |
virion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Gan, L et al. (2006) Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM. Structure 14 1655-65 PubMed GONUTS page
- ↑ Duda, RL et al. (1995) Structural transitions during bacteriophage HK97 head assembly. J. Mol. Biol. 247 618-35 PubMed GONUTS page
- ↑ Oh, B et al. (2014) The delta domain of the HK97 major capsid protein is essential for assembly. Virology 456-457 171-8 PubMed GONUTS page
- ↑ Wikoff, WR et al. (2000) Topologically linked protein rings in the bacteriophage HK97 capsid. Science 289 2129-33 PubMed GONUTS page
- ↑ Veesler, D et al. (2014) Architecture of a dsDNA viral capsid in complex with its maturation protease. Structure 22 230-7 PubMed GONUTS page
- ↑ Veesler, D et al. (2012) Maturation in action: CryoEM study of a viral capsid caught during expansion. Structure 20 1384-90 PubMed GONUTS page
- ↑ Gertsman, I et al. (2009) An unexpected twist in viral capsid maturation. Nature 458 646-50 PubMed GONUTS page
- ↑ Lee, KK et al. (2008) Virus capsid expansion driven by the capture of mobile surface loops. Structure 16 1491-502 PubMed GONUTS page
