Public
Assessment | Ivanerill | 2018-04-15 14:48:30 CDT | No notes given. | Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
|
Public
Assessment | DanielRenfro | 2018-03-31 08:54:04 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0046797 |
viral procapsid maturation |
PMID:24889236 |
IMP: Inferred from Mutant Phenotype |
|
P |
HK97 delta domain is necessary for proper procapsid assembly. HK97 delta domain comprises the first 102 amino acids of the major capsid protein, is present during procapsid assembly, and is cleaved during capsid maturation. In Figure 2A, HK97 lacking the delta domain was found to have become insoluble. Figure 2B shows similar results, with delta-less HK97 present only in the pellet fraction (as marked by a circle). Figure 2C shows expression of a full-length HK97 protein plasmid (protease-) and delta-less HK97. Full-length HK97 is shown to properly form capsomers and proheads—signs of proper assembly— while the lack of bands for the delta-less HK97 show there is no formation of capsomers or proheads. From this, we can conclude that the delta-domain of HK97 is necessary for formation of a properly folded, soluble procapsid head. |
complete
CACAO 13109
| on BPHK7:CAPSD
| Flagged
|
Public
Assessment | Ivanerill | 2018-03-28 10:32:34 CDT | You changed the annotation to cellular component, for which there is no evidence. Look at other BP terms as indicated in the previous assessment.
| Requires Changes
✗ Go term
|
Public
Assessment | DanielRenfro | 2018-03-24 07:56:39 CDT | This annotation has been flagged because it has been edited since last assessment
| Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0046729 |
viral procapsid |
PMID:24889236 |
IMP: Inferred from Mutant Phenotype |
|
C |
HK97 delta domain is necessary for proper procapsid assembly. HK97 delta domain comprises the first 102 amino acids of the major capsid protein, is present during procapsid assembly, and is cleaved during capsid maturation. In Figure 2A, HK97 lacking the delta domain was found to have become insoluble. Figure 2B shows similar results, with delta-less HK97 present only in the pellet fraction (as marked by a circle). Figure 2C shows expression of a full-length HK97 protein plasmid (protease-) and delta-less HK97. Full-length HK97 is shown to properly form capsomers and proheads—signs of proper assembly— while the lack of bands for the delta-less HK97 show there is no formation of capsomers or proheads. From this, we can conclude that the delta-domain of HK97 is necessary for formation of a properly folded, soluble procapsid head. |
complete
CACAO 13109
| on BPHK7:CAPSD
| Flagged
|
Public
Assessment | Ivanerill | 2018-03-23 05:28:03 CDT | The annotation is overall correct. Please evaluate whether a more specific GO term is more appropriate to what is reported in the manuscript.
| Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
|
