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YEREN:Q9F4H2
Contents
| Species (Taxon ID) | Yersinia enterocolitica. (630) | |
| Gene Name(s) | pspC (ECO:0000313 with EMBL:AAG22115.1) | |
| Protein Name(s) | Phage shock protein C (ECO:0000313 with EMBL:AAG22115.1) | |
| External Links | ||
| UniProt | Q9F4H2 | |
| EMBL | AY008264 | |
| IntAct | Q9F4H2 | |
| eggNOG | ENOG4105PY7 COG1983 | |
| GO | GO:0016021 GO:0042802 | |
| InterPro | IPR014320 IPR007168 | |
| Pfam | PF04024 | |
| TIGRFAMs | TIGR02978 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0009898 |
cytoplasmic side of plasma membrane |
ECO:0000314 |
C |
This study about PspC topology allows authors to propose a new working model for activation of the Psp system. Although there have been conflicting past studies on the topology of the shock protein PspC, through multiple direct assays and an in silico analysis with TOPCON, the server for membrane protein topology prediction, the study shows that both the N and C termini of the protein are located in the cytoplasm. The C terminus, which might be the recognition site for FtsH protease and an interaction interface with PspA, would function in the cytoplasm, not the periplasm. In the assay with chemical modification of cysteine residues, seen in Figure 4, CpxP is shown to be located in the periplasm and confirms that 3-maleimidylpropionyl biocytin (MPB) can cross the outer membrane, as in Figure 4A. All PspB cysteine substitution mutants were labeled by MPB only after cell disruption, agreeing with its C terminus location in the cytoplasm, as in Figure B, which is information that has been supported well before. However, all of the PspC cysteine substitution mutants were also labeled only after sonication, whether the cysteine was in the N or C terminus or not, which is shown by the image 4C. This confirms that both ends of PspC are on the cytoplasmic side of the membrane. |
complete | |||||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042802 |
identical protein binding |
ECO:0000353 |
physical interaction evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000323 |
imported automatically asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000323 |
imported automatically asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Flores-Kim, J & Darwin, AJ (2012) Phage shock protein C (PspC) of Yersinia enterocolitica is a polytopic membrane protein with implications for regulation of the Psp stress response. J. Bacteriol. 194 6548-59 PubMed GONUTS page
- ↑ Gueguen, E et al. (2011) The Yersinia enterocolitica phage shock proteins B and C can form homodimers and heterodimers in vivo with the possibility of close association between multiple domains. J. Bacteriol. 193 5747-58 PubMed GONUTS page
