VIBCH:Q9ZFP7

Species (Taxon ID)	Vibrio cholerae. (666)
Gene Name(s)	chiA
Protein Name(s)	Endochitinase ChiA
External Links
UniProt	Q9ZFP7
EMBL	AF097314
ProteinModelPortal	Q9ZFP7
CAZy	CBM5 GH18
GO	GO:0005576 GO:0030246 GO:0043169 GO:0004553 GO:0005975
InterPro	IPR003610 IPR001223 IPR001579 IPR013781 IPR017853
Gene3D	G3DSA:2.10.10.20 G3DSA:3.20.20.80
Pfam	PF02839 PF00704
SMART	SM00495
SUPFAM	SSF51055 SSF51445
PROSITE	PS01095

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0005576	extracellular region	PMID:9791107	ECO:0000314			C		The section entitled "Extracellular transport of ChiA" reports that the semiquantative densitometric analysis of wild type Vibrio cholerae 569B culture was consistent with extracellular secretion of the protein. Figure 7 shows the localization distribution between the culture supernatant and the periplasmic space. A Vibrio cholerae mutant, M14, is also in Figure 7 but it is not relevant to this annotation. Additionally, immunoblot assays were performed to detect the immunoreactive ChiA polypeptides used in the semiquantative densitometric analysis. Therefore, this annotation has the evidence code IDA and not IMP.	complete
	GO:0016798	hydrolase activity, acting on glycosyl bonds	PMID:9791107	ECO:0000314			F		The section entitled "Defining the substrate specificity ChiA" reported that the biochemical assay performed to detect chitinase activity of ChiA was consistent with the hydrolysis of glycosidic bonds. Table 2 shows the results of the assay for determining the substrate specificity of ChiA in wild type Vibrio cholerae as well as ChiA from strains of Escherichia coli that have plasmids encoding the chiA gene.	complete
	GO:0008843	endochitinase activity	PMID:9791107	ECO:0000315			F		Figure 3 is a photograph of the growth of the Vibrio cholerae wild type strain 569B and mutant strain 569BchiA::kan. The growth medium is Luria-Bertani agar with ethylene glycol chitin as a source of chitin. The cloudy ring around the wild type strain indicates a zone of clearing due to endochitinase activity.	complete
	GO:0004568	chitinase activity	PMID:9791107	ECO:0000314			F		Figure 1 is a photograph of the growth of the Vibrio cholerae wild type strain 569B after 24 hours of incubation. The growth medium is Luria-Bertani agar with ethylene glycol chitin as a source of chitin. The cloudy ring around the wild type strain indicates a zone of clearing due to chitinase activity.	complete
enables	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001579 InterPro:IPR003610 InterPro:IPR036573	F		Seeded From UniProt	complete
part_of	GO:0005576	extracellular region	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR003610 InterPro:IPR036573	C		Seeded From UniProt	complete
involved_in	GO:0005975	carbohydrate metabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR001223 InterPro:IPR001579 InterPro:IPR003610 InterPro:IPR036573	P		Seeded From UniProt	complete
enables	GO:0008061	chitin binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR011583	F		Seeded From UniProt	complete
enables	GO:0030246	carbohydrate binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR003610 InterPro:IPR036573	F		Seeded From UniProt	complete
enables	GO:0016798	hydrolase activity, acting on glycosyl bonds	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
involved_in	GO:0008152	metabolic process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	P		Seeded From UniProt	complete
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References

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