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User:Wgrant

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UMBC Phage Hunters Spring 2015

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBPT4:D9IER52015-04-08 09:35:22 CDTGO:0032549 ribonucleoside binding (F)PMID:809436ECO:0000314 direct assay evidence used in manual assertion

Addition of 1M guanidine HCL cause dissociation of proteins B1 and B2. B2 protein recovered was enzymatically inactive until treatment with iron. Preparations of B2 containing 2.3g and 2.1g atoms of iron per mol and an excess of active B1 both showed ribonucleotide reductase activity yielded 315nM and 280nM of NADPH respectively. This can be observed in Fig4.

The molecular activity of native reductase is between 200nm and 300nm. Also, solutions containing inactive B2 without iron had 2% the activity of iron treated B2.

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