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BPT4:D9IER5

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Species (Taxon ID) Enterobacteria phage T4 (Bacteriophage T4). (10665)
Gene Name(s) nrdB (ECO:0000313 with EMBL:ADJ39951.1)
Protein Name(s) Aerobic NDP reductase small subunit (ECO:0000313 with EMBL:ADJ39951.1)
External Links
UniProt D9IER5
EMBL HM137666
KJ477684
KJ477685
KJ477686
RefSeq NP_049841.1
ProteinModelPortal D9IER5
GeneID 1258601
Proteomes UP000001092
GO GO:0016491
GO:0009186
Gene3D 1.10.620.20
InterPro IPR009078
IPR012348
IPR000358
PANTHER PTHR23409
Pfam PF00268
SUPFAM SSF47240
PROSITE PS00368

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

enables

GO:0032549

ribonucleoside binding

PMID:809436[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0009263

deoxyribonucleotide biosynthetic process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR033909

P

Seeded From UniProt

complete

GO:0032549

ribonucleoside binding

PMID:809436[1]

ECO:0000314

F

Addition of 1M guanidine HCL cause dissociation of proteins B1 and B2. B2 protein recovered was enzymatically inactive until treatment with iron. Preparations of B2 containing 2.3g and 2.1g atoms of iron per mol and an excess of active B1 both showed ribonucleotide reductase activity yielded 315nM and 280nM of NADPH respectively. This can be observed in Fig4.

The molecular activity of native reductase is between 200nm and 300nm. Also, solutions containing inactive B2 without iron had 2% the activity of iron treated B2.

complete
CACAO 10896

enables

GO:0016491

oxidoreductase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR012348

F

Seeded From UniProt

complete

involved_in

GO:0055114

oxidation-reduction process

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000358
InterPro:IPR012348

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Berglund, O (1975) Ribonucleoside diphosphate reductase induced by bacteriophage T4. III. Isolation and characterization of proteins B1 and B2. J. Biol. Chem. 250 7450-5 PubMed GONUTS page