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User:Waldhorn

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableRHOJR:Q932V12012-04-10 11:31:33 CDTGO:0043639 benzoate catabolic process (P)PMID:11673430ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 6 indicates that the wildtype RHA1 is able to degrade benzoate, a product of biphenyl metabolism, as indicated by a final concentration of 0mM benzoate in the medium. The mutant, RDB169 (benA deleted), is unable to degrade the benzoate as effectively as shown by final benzoate concentrations between 3.3 and 6.8mM in the medium.

challenge
requireschangesFLAJ1:A5FIT12012-04-15 12:02:19 CDTGO:0071976 cell gliding (P)PMID:20038590ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2A and 2E shows that wildtype cells form spreading colonies whereas cells of the gldN mutant formed nonspreading colonies.

challenge
requireschangesFLAJ1:A5FIT22012-04-15 12:06:35 CDTGO:0071976 cell gliding (P)PMID:20038590ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 2A and 2I show that wildtype cells form spreading colonies whereas cells of the glnO mutant formed nonspreading colonies

challenge
acceptableFLAJ1:A1E5U52012-04-15 12:37:16 CDTGO:0071976 cell gliding (P)PMID:21421754ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 9A and B shows that the wildtype cells glide successfully whereas the sprB deletion mutant glides hardly at all.

challenge
requireschangesFLAJ1:A1E5T92012-04-15 12:52:11 CDTGO:0071976 cell gliding (P)PMID:21784937ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that the average number of cells attached to 0.03mm^2 region of glass coverslip was 52.9 whereas the sprE deficient mutant only had 2 cells attached for the same area. This is reiterated in Fig. 2 where the wildtype has spreading colonies and the sprE mutant does not produce spreading colonies.

challenge
unacceptableASPFM:Q6S9W42012-04-15 13:17:24 CDTGO:0030010 establishment of cell polarity (P)PMID:19389762ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4 shows that the wildtype has an accumulation of actin patches at the poles whereas the mutant has actin patches evenly distributed throughout.

challenge

acceptable:2
unacceptable:1
requires_changes:3
flagged:0

Annotations challenged by Waldhorn

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Waldhorn


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschanges9GAMM:A5H9N62012-03-31 22:29:46 CDTGO:0043448 alkane catabolic process (P)PMID:17400787ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 indicates that almA deficient mutants produce little to no protein when grown in medium containing alkanes with chain length greater than 32 whereas the wildtype is able to successfully produce protein when grown on medium containing these long chain alkanes. almA encodes AlmA, a putative flavin-binding monooxygenase, which is involved in metabolism of long chain alkanes.

challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Waldhorn

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Waldhorn