GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:21784937
| Citation |
Rhodes, RG, Samarasam, MN, Van Groll, EJ and McBride, MJ (2011) Mutations in Flavobacterium johnsoniae sprE result in defects in gliding motility and protein secretion. J. Bacteriol. 193:5322-7 |
|---|---|
| Abstract |
Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Transposon mutagenesis was used to identify sprE, which is involved in gliding. Mutations in sprE resulted in the formation of nonspreading colonies on agar. sprE mutant cells in wet mounts were almost completely deficient in attachment to and movement on glass, but a small percentage of cells exhibited slight movements, indicating that the motility machinery was not completely disrupted. SprE is a predicted lipoprotein with a tetratricopeptide repeat domain. SprE is similar in sequence to Porphyromonas gingivalis PorW, which is required for secretion of gingipain protease virulence factors. Disruption of F. johnsoniae sprE resulted in decreased extracellular chitinase activity and decreased secretion of the cell surface motility protein SprB. Reduced secretion of cell surface components of the gliding machinery, such as SprB, may account for the defects in gliding. Orthologs of sprE are found in many gliding and nongliding members of the phylum Bacteroidetes, suggesting that similar protein secretion systems are common among members of this large and diverse group of bacteria. |
| Links |
PubMed PMC3187464 Online version:10.1128/JB.05480-11 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Blotting, Western; Chitinase/genetics; Chitinase/metabolism; Flavobacterium/genetics; Flavobacterium/physiology; Lipoproteins/genetics; Lipoproteins/metabolism; Molecular Sequence Data; Mutation |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0071976: cell gliding |
ECO:0000315: |
P |
Table 1 shows that the average number of cells attached to 0.03mm^2 region of glass coverslip was 52.9 whereas the sprE deficient mutant only had 2 cells attached for the same area. This is reiterated in Fig. 2 where the wildtype has spreading colonies and the sprE mutant does not produce spreading colonies. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
