User:Swiftell

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	MENSP:Q40322	2012-04-14 10:30:25 CDT	GO:0034002 (R)-limonene synthase activity (F)	PMID:8226816	ECO:0000315 mutant phenotype evidence used in manual assertion	The amino acid sequence of limonene synthase was purified out of spearmint leaf and put into cDNA. Fig. 4 pLC 5.2 cDNA gave the highest specific activity of limonene synthase expression, which confirms the cDNA to represent this gene.	challenge
requireschanges	OCIBA:GEDH1	2012-04-14 12:30:41 CDT	GO:0047924 geraniol dehydrogenase activity (F)	PMID:16150417	ECO:0000314 direct assay evidence used in manual assertion	Fig. 5 shows geraniol oxidation by CAD1 or GEDH1 to produce geranial.	challenge
requireschanges	ECOLI:SYE	2012-04-14 12:54:43 CDT	GO:0004818 glutamate-tRNA ligase activity (F)	PMID:6280993	ECO:0000314 direct assay evidence used in manual assertion	Table 4 and Fig. 4 shows that GluRS can bind both ATP and its tRNA substrate independently, but binding of glutamate requires the presence of tRNAGlu.	challenge
requireschanges	ECOLI:HEM3	2012-04-14 13:25:28 CDT	GO:0004418 hydroxymethylbilane synthase activity (F)	PMID:1747120	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 2 shows a liquid chromatography column of mutants. Fig. 2b specifically shows that the RH11 mutant cannot create the enzyme-intermediate needed to catalyze the reaction.	challenge

acceptable:0
unacceptable:0
requires_changes:4
flagged:0

Annotations challenged by Swiftell

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Ortizvi3, Team Las Transposones	LACCA:Q840T2	GO:0034338 - short-chain carboxylesterase activity (F)	PMID:15184114	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Swiftell

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	PSESP:AMNE	2012-03-29 12:41:20 CDT	GO:0047437 4-oxalocrotonate decarboxylase activity (F)	PMID:11081795	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 (b) shows the enzyme AmnE, which is 4-oxalocrotonate decarboxylase, catalyzing the reaction from 4-oxalocrotinic acid to 2-oxopent-4-enoic acid. The enzyme was assayed spectrophotometrically using the substrates listed above.	challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Swiftell

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Ortizvi3, Team Las Transposones	LACCA:Q840T2	GO:0034338 - short-chain carboxylesterase activity (F)	PMID:15184114	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Swiftell