PMID:11081795

Citation	Takenaka, S, Murakami, S, Kim, YJ and Aoki, K (2000) Complete nucleotide sequence and functional analysis of the genes for 2-aminophenol metabolism from Pseudomonas sp. AP-3. Arch. Microbiol. 174:265-72
Abstract	A 13.9-kb region, which contained the 2-aminophenol 1,6-dioxygenase genes (amnBA) reported before, was cloned from the 2-aminophenol-assimilating bacterium Pseudomonas sp. AP-3. The complete nucleotide sequence of this region was determined and six genes were found downstream of amnBA. The eight genes together were designated amnBACFEDHG. Each gene was similar to the corresponding gene operating in the meta-cleavage pathway, except for amnB, amnA, and amnD. The four 2-aminophenol-metabolizing enzymes, 2-aminomuconic 6-semialdehyde dehydrogenase, 2-aminomuconate deaminase, 4-oxalocrotonate decarboxylase, and 2-oxopent-4-enoate hydratase, were purified and characterized. NH2-terminal amino acid sequences of each purified enzyme agreed with those deduced from amnC, amnF, amnE, and amnD, respectively. These genes were therefore assigned as the genes encoding these respective proteins. The tight clustering of the amn genes, which were all transcribed in the same direction, raised the possibility that these genes formed a single operon. The organization of the amn genes was entirely different from that of the atd, dmp, and xyl genes reported in the meta-cleavage pathway, although these latter genes clustered similarly.
Links	PubMed
Keywords	Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/isolation & purification; Aldehyde Oxidoreductases/metabolism; Aminohydrolases/genetics; Aminohydrolases/isolation & purification; Aminohydrolases/metabolism; Aminomuconate-Semialdehyde Dehydrogenase; Aminophenols/metabolism; Base Sequence; Carboxy-Lyases/genetics; Carboxy-Lyases/isolation & purification; Carboxy-Lyases/metabolism; Cloning, Molecular; Dioxygenases; Hydro-Lyases/genetics; Hydro-Lyases/isolation & purification; Hydro-Lyases/metabolism; Molecular Sequence Data; Operon; Oxygenases/genetics; Oxygenases/metabolism; Pseudomonas/enzymology; Pseudomonas/genetics; Sequence Analysis, DNA
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PSESP:AMNE	NOT	GO:0047437: 4-oxalocrotonate decarboxylase activity	ECO:0000314:		F		Figure 1 (b) shows the enzyme AmnE, which is 4-oxalocrotonate decarboxylase, catalyzing the reaction from 4-oxalocrotinic acid to 2-oxopent-4-enoic acid. The enzyme was assayed spectrophotometrically using the substrates listed above.	complete CACAO 4078

References

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