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User:Shea0003

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CACAO Fall 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableBACPE:A7LKG52014-10-26 16:58:07 CDTGO:0055114 oxidation-reduction process (P)PMID:18359284ECO:0000314 direct assay evidence used in manual assertion

Oxidation Reduction Process

Figure 3b pBAD-OF4-Mrp was used as positive control and showed antiport activity

challenge
updatedbyinstructorBACPE:A7LKG52014-11-09 17:33:54 CSTGO:0050136 NADH dehydrogenase (quinone) activity (F)PMID:18359284ECO:0000314 direct assay evidence used in manual assertion

Table 2, shows kinetic parameters of NDH2-2 enzyme of various substrates

challenge
unacceptableBACHD:Q9K7F42014-11-21 16:19:37 CSTGO:0050660 flavin adenine dinucleotide binding (F)PMID:11058132ECO:0000314 direct assay evidence used in manual assertion

figure 2 shows distribution of A+T-rich islands along the chromosome indicates flavin adenine dinucleotide binding

challenge
unacceptableBACPE:A7LKG42014-11-23 17:04:04 CSTGO:0050660 flavin adenine dinucleotide binding (F)PMID:18359284ECO:0000314 direct assay evidence used in manual assertion

Figure 5e lane 2 shows positive analysis for purified protein. Absorbance differences also seen in figures 5a and c

challenge
requireschangesHUMAN:FFAR42014-11-23 17:46:25 CSTGO:0046879 hormone secretion (P)PMID:15619630ECO:0000314 direct assay evidence used in manual assertion

Figure 2A diplays FFA-induced GLP-1 secretion from STC-1 cells

challenge
unacceptableHUMAN:FFAR42014-11-23 17:46:25 CSTGO:0046879 hormone secretion (P)PMID:15619630ECO:0000314 direct assay evidence used in manual assertion

Figure 3 displays expression of Gpr120, but not Gpr40, which is mediated by FFA-induced calcium concentration response and GLP-1 secretion.

challenge
unacceptableHUMAN:FFAR42014-11-23 17:46:25 CSTGO:0046879 hormone secretion (P)PMID:15619630ECO:0000314 direct assay evidence used in manual assertion

Figure 4 c, d, displays effects of alpha-linolenic acids intubation on plasma GLP-1 and insulin concentrations in portal vein and IVC of mice.

challenge

acceptable:0
unacceptable:5
requires_changes:1
flagged:0

Annotations challenged by Shea0003

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0 annotations fixed by Shea0003

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