User:Quennev3

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	YEASA:E7KD91	2013-04-14 15:38:19 CDT	GO:0006021 inositol biosynthetic process (P)	PMID:23578934	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 8 shows overproduction of inositol in an Opi1 mutant.	challenge
correctedthroughchallenges	YEAST:LSB1	2013-04-14 17:20:08 CDT	GO:0030837 negative regulation of actin filament polymerization (P)	PMID:23577202	ECO:0000021 physical interaction evidence	Figure 2 gives evidence of Lsb1 and Lsb2 inhibiting Las17. Figure 4 shows Lsb1 and Lsb2 are colocalized with Las17, a protein involved in actin polymerization.	challenge
requireschanges	YEAST:SLA1	2013-04-14 17:35:12 CDT	GO:0030833 regulation of actin filament polymerization (P)	PMID:22973053	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows that Sla1 and Las17 arrive and disperse simultaneously during endocytosis and form a stable complex in the cytosol; this was observed using microscopy. Figure 3 shows use of a GST fusion affinity assay to demonstrate interaction between Sla1 and Las17 is "direct and strong".	challenge
requireschanges	YEAST:RSP5	2013-04-14 17:53:17 CDT	GO:0006890 retrograde vesicle-mediated transport, Golgi to ER (P)	PMID:22761830	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows Rsp4 mutants have impaired retrograde trafficking from Golgi to ER. Figure 7 shows similar evidence.	challenge

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Annotations challenged by Quennev3

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