UMBC Phage Hunters Spring 2015
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | 9CAUD:S5YPI2 | 2015-04-08 08:55:51 CDT | GO:0003677 exonuclease activity (F) | GO_REF:0000100 | ECO:0000247 sequence alignment evidence used in manual assertion | An HHPred job of S5YPI2 provides evidence that G4FG24 and S5YPI2 have sequence alignment.
BLASTP: BLAST YP_008430900.1 (S5YPI2) YP_007977993 (Q9X1X0) is a result with E-value of 2e-10, score of 70.9, query coverage of 91%, 26% identity.
HHPred: The protein sequence of S5YPI2 returns the first result 3THO which is part of Q9X1X0 (Score: 296.59; E-value: 2e-38; Similarity: 0.301; % Identity: 23)
Protein may be dual exonuclease/metallophosphatase with DNA-binding activity.
| challenge |
| acceptable | THEMA:Q9X1X0 | 2015-05-11 16:29:57 CDT | GO:0003677 exonuclease activity (F) | PMID:21937514 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 4: A comparison of DNA affinity for two versions of the proposed exonuclease using EMSA (elecrophoretic mobility shift assay). When starved of ATP, TmMRE loses affinity for DNA binding. MRE requires dimerization followed by ATP hydrolysis to perform exonuclease activity.
Figure 5: Schematic of DNA binding mechanism via the Antibody DNA-binding Assay.
These results allowed the researchers to conclude with confidence taht Q9X1X0 is a DNA-binding and exonuclease protein.
| challenge |
acceptable:2
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by Njulie
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Njulie
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