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CACAO Phage Hunters 2019

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StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableTREPA:TA172019-03-26 22:08:31 CDTGO:0005886 plasma membrane (C)PMID:27161310ECO:0007103 epitope-tagged protein immunolocalization evidence used in manual assertion

Figure 1 displays the results of Indirect Flourescent Antibody assays (IFA) conducted on two B. burgdorferi strains, one containing an empty vector and the other containing a TP0435 vector. In columns A and C, the surfaces of both strains were stained while in columns B and D, the membranes of both strains were permeabilized before staining. Both vector strains were labeled more frequently than the control strains, indicating that the addition of the vector resulted in the expression of TP0435. In addition, permeabilization of the vector strain membrane resulted in significantly more staining than the nonpermeable vector strain, indicating that TP0435 was more frequently present on the periplasmic membrane surface.



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