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TREPA:TA17
Contents
| Species (Taxon ID) | Treponema pallidum (strain Nichols). (243276) | |
| Gene Name(s) | tpp17 | |
| Protein Name(s) | 17 kDa lipoprotein | |
| External Links | ||
| UniProt | P29722 | |
| EMBL | M74825 AE000520 | |
| PIR | C71323 | |
| RefSeq | WP_010881883.1 | |
| PDB | 4U3Q | |
| PDBsum | 4U3Q | |
| ProteinModelPortal | P29722 | |
| SMR | P29722 | |
| IntAct | P29722 | |
| STRING | 243276.TP0435 | |
| EnsemblBacteria | AAC65423 | |
| GeneID | 34332383 | |
| KEGG | tpa:TP_0435 | |
| eggNOG | COG3015 | |
| OMA | GNSVRYM | |
| OrthoDB | 2029962at2 | |
| Proteomes | UP000000811 | |
| GO | GO:0005886 | |
| InterPro | IPR007298 | |
| Pfam | PF04170 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005886 |
plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
||||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
||||
|
part_of |
GO:0005886 |
plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
||||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
||||
|
part_of |
GO:0005886 |
plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
||||
|
Colocalizes with |
GO:0005886 |
plasma membrane |
ECO:0005592 |
immunogold labelling electron microscopy assay evidence used in manual assertion |
C |
In Figure 3, Immuno-SEM, specifically using immunogold labeling, was utilized to analyze the position of TP0435 (also known as gene tpp17) on the plasma membrane. There was not much gold/antibiotic tagging when the outside of the B. burgdorferi cell was labeled. In Figure 4, immunogold labeling was utilized again to show that the tpp17 structural protein is much more present in the inner, periplasmic side of the outer plasma membrane of the B. burgdorferi cell. |
complete | |||
|
Colocalizes with |
GO:0005886 |
plasma membrane |
ECO:0007103 |
epitope-tagged protein immunolocalization evidence used in manual assertion |
C |
Figure 1 displays the results of Indirect Flourescent Antibody assays (IFA) conducted on two B. burgdorferi strains, one containing an empty vector and the other containing a TP0435 vector. In columns A and C, the surfaces of both strains were stained while in columns B and D, the membranes of both strains were permeabilized before staining. Both vector strains were labeled more frequently than the control strains, indicating that the addition of the vector resulted in the expression of TP0435. In addition, permeabilization of the vector strain membrane resulted in significantly more staining than the nonpermeable vector strain, indicating that TP0435 was more frequently present on the periplasmic membrane surface. |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Chan, K et al. (2016) Treponema pallidum Lipoprotein TP0435 Expressed in Borrelia burgdorferi Produces Multiple Surface/Periplasmic Isoforms and mediates Adherence. Sci Rep 6 25593 PubMed GONUTS page
