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User:Jknapo1

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Phage Hunters Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorBPT5:HEL102017-04-05 10:33:09 CDTGO:0008094 DNA-dependent ATPase activity (F)PMID:28009009ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1b shows the mass of the eluted molecules to be consistent with those in DNA-dependent ATPase activity. Figure 1c shows enzymatic activity was only stimulated in the presence of duplex DNA. Figure 1d shows after cloning of D10 protein, D10-R389N (motif VI) was expressed and purified. When compared to wild type, a point mutation of the motif stopped all DNA-dependent ATPase activity.

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