Phage Hunters Spring 2017
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
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| BPR69:Q7Y5B1 | 2017-04-30 22:44:42 CDT | GO:0039624 viral outer capsid (C) | PMID:19835886 | ECO:0000255 match to sequence model evidence used in manual assertion | Figure 6a shows how the structure of the RB69 soc protein fits into a cryo EM model of the T4 capsid through trimeric interactions, where the major capsid protein gp23 of T4 has a 94% sequence identity to the major capsid protein of RB69. Fitting was done through the use of the EMFIT program, originally developed by Michael Rossmann (2000) (PMID:10998631). The stereo diagrams of the protein structure was derived from the RESOLVE program shown in Figure 2b using the amino acid sequence shown in Figure 2a. Figure 7b shows how six trimers of the soc (shown in red) assemble around each major capsid protein gp23 hexamer (each shown in blue, black, green, or magenta), acting to stabilize the connection between each capsid protein, a key characteristic of an outer capsid protein. A larger view that shows the stabilizing cage of the whole phage capsid can be seen in Figure 7a, with the red representing the soc proteins.
| challenge |
| acceptable | BPP22:CAPSD | 2017-04-26 08:50:34 CDT | GO:0039620 T=7 icosahedral viral capsid (C) | PMID:28270620 | ECO:0000314 direct assay evidence used in manual assertion | Gp5 of Salmonella phage P22 was shown to fold into a classical HK97 capsid fold (PMID:11000116). Figure 1b shows a density map of the P22 capsid cryo-EM model. From the cryo-EM image, one asymmetrical unit, outlined in red in Figure 1b, was modeled and presented in Figure 1c with seven different colors representing each of the seven proteins folding into a T=7 icosahedral structure. This cryo-EM derived model of the protein matched closely to the molecular model of the gp5 430 amino acid sequence shown in Figure 2b. This 430 amino acid sequence number corresponds to the BLAST sequence in Uniprot for gp5, confirming that the model derived from the cryo-EM imaging was indeed gp5.
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acceptable:1
unacceptable:0
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Annotations challenged by Hueydj
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
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