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CACAO Phage Hunters 2018

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StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBPSPP:TUBE2018-03-31 22:28:56 CDTGO:0098026 virus tail, tube (C)PMID:25525268ECO:0000314 direct assay evidence used in manual assertion

TTP, gp17.1 has been identified to be able to self assemble into long tubes in the absence of other phage proteins. In figure 6 there is a comparison done between gp17.1 and tails of SSP1 virions that show similar morphology. These samples were negatively stained and it was observed that both gp17.1 is hollow and the empty virion tails that have ejected their DNA after incubation in a SSP1 bacterial receptor. Aligned segments of gp17.1 were taken then calculation was performed for its diffraction pattern. This pattern revealed that tubes have a 6-fold symmetry which are made by stacks of hexameric rings. These rings form helical tubes that elongate.

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