Phage Hunters Spring 2016
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| unacceptable | BPT5:LLP | 2016-04-21 02:05:41 CDT | GO:0019085 early viral transcription (P) | PMID:8057856 | ECO:0000314 direct assay evidence used in manual assertion | Figure 8 shows the expression of T5 proteins during infection of E. coli F cells. All proteins were separated on a SDS-polyacrylamide gel and samples were analyzed at six time points. Llp was synthesized 5 minutes after infection and Llp showed the most expression at 10 minutes. In later time point, Llp was expressed less. This suggests that Llp was produced during early transcription.
| challenge |
| acceptable | BPT5:LLP | 2016-04-21 02:05:44 CDT | GO:1902735 negative regulation of receptor-mediated virion attachment to host cell (P) | PMID:8057856 | ECO:0000315 mutant phenotype evidence used in manual assertion | The data from Table 2 suggested that Llp blocks the adsorption of phage T5 to the FhuA receptor protein on the host cell membrane. T5 adsorption was carried out for 10 minutes on medium plates at 37 deg C. T5 adsorption was measured by using standard cell and phage concentrations from another experiment. For JM101/(pVK3 – vector with no mutations and carries functional oad and llp genes), 82% of phages were not adsorbed to FhuA. For JM 101/(pVK3/IS1-2 – vector with an insertion downstream of oad and llp genes), 85% of phages were not adsorbed to FhuA. JM101/(pLG339 – vector with no llp gene), only 12% of phages were not adsorbed. This data suggests that llp blocks adsorption of T5 to FhuA.
| challenge |
| acceptable | BPT5:LLP | 2016-04-21 02:05:45 CDT | GO:0036406 anchored component of periplasmic side of cell outer membrane (C) | PMID:8057856 | ECO:0000314 direct assay evidence used in manual assertion | Figure 7B shows that globomycin inhibits the processing of lipoproteins which is shown as no expression of llp (no bands) at 20 ug/ml and 120 ug/ml. At 5 ug/ml globomycin, processing of lipoprotein still occurs but is still inhibited since there is still expression of precursor lipoprotein (p-Llp). With no globomycin, Llp is processed as shown with a band near the 8kDa marker but no expression at around 9 kDa. This suggests that Llp is a lipoprotein and must be processed in order to bind to the FhuA protein on the periplasmic side of the cell outer membrane.
| challenge |
| unacceptable | 9CAUD:I1TE39 | 2016-05-06 16:52:26 CDT | GO:0098024 virus tail, fiber (C) | PMID:24198424 | ECO:0000314 direct assay evidence used in manual assertion | Figure 1 shows the SDS-PAGE of T5 protein components including a straight tail fiber (pb4). The SDS-PAGE shows that the molecular weight of the straight tail fiber protein (pb4) to be around the 75 kDa marker.
| challenge |
| updatedbyinstructor | BPT5:BPPB3 | 2016-05-06 16:57:27 CDT | GO:0098015 virus tail (C) | PMID:24198424 | ECO:0000314 direct assay evidence used in manual assertion | Figure 1 shows the SDS-PAGE of T5 protein components including pb3 protein. Since pb3 is one of the larger proteins that make the viral tail complex, the SDS-PAGE shows that pb3 has a high molecular weight of more than 75 kDa than other tail protein components.
| challenge |
| unacceptable | BPT5:DIT | 2016-05-06 17:00:11 CDT | GO:0098020 virus tail, minor subunit (C) | PMID:24198424 | ECO:0000314 direct assay evidence used in manual assertion | Figure 1 shows the SDS-PAGE of T5 protein components. DIT is a distal tail protein which is a small component of a tail tip protein complex. Figure shows that pb9 has a low molecular weight around 25 kDa.
| challenge |
acceptable:2
unacceptable:3
requires_changes:0
flagged:0
Annotations challenged by ArmandoMaruffo
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by ArmandoMaruffo
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