GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

BPT5:LLP

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Enterobacteria phage T5 (Bacteriophage T5). (10726)
Gene Name(s) llp
Protein Name(s) Lytic conversion lipoprotein
External Links
UniProt Q38162
EMBL X75922
AY543070
PIR S46547
RefSeq YP_006986.1
GeneID 2777682
Proteomes UP000002107
GO GO:0033644
GO:0016020

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0031225

anchored to membrane

PMID:8057856[1]

ECO:0000314

C

Fig. 7.

complete

GO:0019085

early viral transcription

PMID:8057856[1]

ECO:0000314

P

Figure 8 shows the expression of T5 proteins during infection of E. coli F cells. All proteins were separated on a SDS-polyacrylamide gel and samples were analyzed at six time points. Llp was synthesized 5 minutes after infection and Llp showed the most expression at 10 minutes. In later time point, Llp was expressed less. This suggests that Llp was produced during early transcription.

complete
CACAO 11868

GO:1902735

negative regulation of receptor-mediated virion attachment to host cell

PMID:8057856[1]

ECO:0000315

P

The data from Table 2 suggested that Llp blocks the adsorption of phage T5 to the FhuA receptor protein on the host cell membrane. T5 adsorption was carried out for 10 minutes on medium plates at 37 deg C. T5 adsorption was measured by using standard cell and phage concentrations from another experiment. For JM101/(pVK3 – vector with no mutations and carries functional oad and llp genes), 82% of phages were not adsorbed to FhuA. For JM 101/(pVK3/IS1-2 – vector with an insertion downstream of oad and llp genes), 85% of phages were not adsorbed to FhuA. JM101/(pLG339 – vector with no llp gene), only 12% of phages were not adsorbed. This data suggests that llp blocks adsorption of T5 to FhuA.

complete
CACAO 11869

GO:0036406

anchored component of periplasmic side of cell outer membrane

PMID:8057856[1]

ECO:0000314

C

Figure 7B shows that globomycin inhibits the processing of lipoproteins which is shown as no expression of llp (no bands) at 20 ug/ml and 120 ug/ml. At 5 ug/ml globomycin, processing of lipoprotein still occurs but is still inhibited since there is still expression of precursor lipoprotein (p-Llp). With no globomycin, Llp is processed as shown with a band near the 8kDa marker but no expression at around 9 kDa. This suggests that Llp is a lipoprotein and must be processed in order to bind to the FhuA protein on the periplasmic side of the cell outer membrane.

complete
CACAO 11870

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

involved_in

GO:0098669

superinfection exclusion

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1260

P

Seeded From UniProt

complete

involved_in

GO:0016032

viral process

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0945

P

Seeded From UniProt

complete

part_of

GO:0033644

host cell membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1043

C

Seeded From UniProt

complete

part_of

GO:0020002

host cell plasma membrane

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1033
UniProtKB-SubCell:SL-0376

C

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 Decker, K et al. (1994) Lytic conversion of Escherichia coli by bacteriophage T5: blocking of the FhuA receptor protein by a lipoprotein expressed early during infection. Mol. Microbiol. 12 321-32 PubMed GONUTS page