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BPT5:LLP
Contents
Species (Taxon ID) | Enterobacteria phage T5 (Bacteriophage T5). (10726) | |
Gene Name(s) | llp | |
Protein Name(s) | Lytic conversion lipoprotein | |
External Links | ||
UniProt | Q38162 | |
EMBL | X75922 AY543070 | |
PIR | S46547 | |
RefSeq | YP_006986.1 | |
GeneID | 2777682 | |
Proteomes | UP000002107 | |
GO | GO:0033644 GO:0016020 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0031225 |
anchored to membrane |
ECO:0000314 |
C |
Fig. 7. |
complete | |||||
GO:0019085 |
early viral transcription |
ECO:0000314 |
P |
Figure 8 shows the expression of T5 proteins during infection of E. coli F cells. All proteins were separated on a SDS-polyacrylamide gel and samples were analyzed at six time points. Llp was synthesized 5 minutes after infection and Llp showed the most expression at 10 minutes. In later time point, Llp was expressed less. This suggests that Llp was produced during early transcription. |
complete | |||||
GO:1902735 |
negative regulation of receptor-mediated virion attachment to host cell |
ECO:0000315 |
P |
The data from Table 2 suggested that Llp blocks the adsorption of phage T5 to the FhuA receptor protein on the host cell membrane. T5 adsorption was carried out for 10 minutes on medium plates at 37 deg C. T5 adsorption was measured by using standard cell and phage concentrations from another experiment. For JM101/(pVK3 – vector with no mutations and carries functional oad and llp genes), 82% of phages were not adsorbed to FhuA. For JM 101/(pVK3/IS1-2 – vector with an insertion downstream of oad and llp genes), 85% of phages were not adsorbed to FhuA. JM101/(pLG339 – vector with no llp gene), only 12% of phages were not adsorbed. This data suggests that llp blocks adsorption of T5 to FhuA. |
complete | |||||
GO:0036406 |
anchored component of periplasmic side of cell outer membrane |
ECO:0000314 |
C |
Figure 7B shows that globomycin inhibits the processing of lipoproteins which is shown as no expression of llp (no bands) at 20 ug/ml and 120 ug/ml. At 5 ug/ml globomycin, processing of lipoprotein still occurs but is still inhibited since there is still expression of precursor lipoprotein (p-Llp). With no globomycin, Llp is processed as shown with a band near the 8kDa marker but no expression at around 9 kDa. This suggests that Llp is a lipoprotein and must be processed in order to bind to the FhuA protein on the periplasmic side of the cell outer membrane. |
complete | |||||
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0098669 |
superinfection exclusion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
involved_in |
GO:0016032 |
viral process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
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