User:Adhikaris9

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
flagged	LAMBD:HOLIN	2017-04-26 10:02:38 CDT	GO:0020002 host cell plasma membrane (C)	PMID:11018145	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1: Lysis requires the genes S and R. R codes for endolysin. S codes for the holin and holin inhibitor (genes 105 and 107) in Lambda. Lambda phages were mutated to be either without the holin gene (S), and with the endolysin (R) gene (or vice versa) or with both holin and endolysin gene. And as seen in the image, the lambda phages without the S gene did not lyse. However, the lambda phages with out R did lyse, at about 60 minuets. And the Lambda phage with both lysed at 40 minuets, which was the quickest lyse without the help of methyl trichloride. This suggests that endolysin is not required for lysis but involved in cleaving the bonds in the peptidoglycan.	challenge

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Annotations challenged by Adhikaris9

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