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GO:0004674protein serine/threonine kinase activityPMID:18442973IDA: Inferred from Direct Assay F
This annotation made on page: CORGL:PKN1
By: Lzeihen (group delta3) on 2011-12-01 18:57:14 CST.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeRdesai525,
Team Texas Aggies
2014-04-13 15:09:39 CDT

Uniprot accession should be Q8NU97 (PKN2_CORGL): Serine/threonine-protein kinases PknA

Go term should be: GO:0004674 (protein serine/threonine kinase activity)

Figure 1 can not be used in CACAO because it's a sequence alignment diagram, thus a more appropriate experimental evidence for serine/threonine kinase activity should be Fig. 6. which clearly shows that over expression of Pnk-A leads to coccoid morphology without any polar growth. And partial depletion of Pnk-A leads to elongated cells twice or three times the size of the wild-type.

As such, the the experimental evidence should be IMP: inferred from Mutant Phenotype.

0
Private
Assessment
Suzialeksander2014-07-02 14:10:33 CDTYou need to be an instructor to view these notes.Unacceptable
Public
Assessment
DanielRenfro2014-04-13 15:09:39 CDT

This annotation has been flagged because it has been edited since last assessment ||GO:0004674||protein serine/threonine kinase activity||PMID:18442973||ISA: Inferred from Sequence Alignment||||F||Figure 1 in text||Missing: with/from

Flagged
Public
Assessment
Bmcintosh2011-12-05 15:32:55 CST

I think this GO term is a bit too specific based on the assay. Do they really show this is a serine/threonine kinase?

Requires Changes
Public
Assessment
Bmcintosh2011-12-05 15:29:30 CST

Annotation is fine. BM

Acceptable