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User:Rdesai525

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableECOK1:MUKB2014-01-28 20:58:22 CSTGO:0051983 regulation of chromosome segregation (P)PMID:24097060ECO:0000314 direct assay evidence used in manual assertion

Figure 4: MukB enhences Topoisomerase IV activity in relexation of negative supercoiled DNA.

challenge
unacceptableNEUCR:KPC12014-02-01 20:11:08 CSTGO:0004697 protein kinase C activity (F)PMID:21070858ECO:0000314 direct assay evidence used in manual assertion

Figure 1. Neurosporacrassa protein kinase C (NPKC) activity is directly enhanced in absence of Choline. This is true for parental strain as well as for the GFP-NPKC transformants.

challenge
unacceptableNEUCR:KPC12014-02-11 20:42:26 CSTGO:1900020 positive regulation of protein kinase C activity (P)PMID:21070858ECO:0000314 direct assay evidence used in manual assertion

Table 2: Generally speaking, as choline concentration increases, conidiation period in neurospora crassa decreases. However, this decrease in conidiation time is not due to an increase in NPKC activity because experimental groups Q and R don't show any significant decrease in conidiation period when compared to wildtype control.

challenge
acceptableNEUCR:KPC12014-03-18 19:45:21 CDTGO:0030428 cell septum (C)PMID:21070858ECO:0000314 direct assay evidence used in manual assertion

Figure 3,cross section of N.crassa septum viewed under confocal microscopy indicates that the PKC is initially localized in the cell septum.

challenge
unacceptableNEUCR:NIT22014-03-18 20:59:47 CDTGO:0044270 cellular nitrogen compound catabolic process (P)PMID:6450203ECO:0000314 direct assay evidence used in manual assertion

Figure 6: nit-2 protein in N.crassa (strain 74-0R23-1A)component of non-histone chromosomal protein is 21,900 daltons.

challenge
acceptableEBVB9:RTA2014-04-01 16:52:45 CDTGO:0050434 positive regulation of viral transcription (P)PMID:17446270ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Rta causes 6 to 33 fold increase in the actively of four target EBV gene promoters.

challenge
acceptableNEUCR:COT12014-04-01 19:49:16 CDTGO:0005886 plasma membrane (C)PMID:10955908ECO:0000314 direct assay evidence used in manual assertion

Figure 2: N.crassa COT-1 kinase is localized in plasma membrane of wild type N.crassa strain.

challenge
acceptableNEUCR:COT12014-04-01 19:49:16 CDTGO:0005886 plasma membrane (C)PMID:10955908ECO:0000315 mutant phenotype evidence used in manual assertion

According to figure 2-B, in comparison to wild type strain, N.Crassa mutant grown at impermissible temperature of 37C has COT1 kinase mostly present in hyphal cytosol and trivial amount of it in plasma membrane.

challenge
acceptableSCHPO:GPI72014-04-05 15:31:36 CDTGO:0005783 endoplasmic reticulum (C)PMID:10641037ECO:0006061 immunofluorescence wide-field microscopy evidence used in manual assertion

According to Figure 3-A, immunofluorescence and microscopy results indicate that GFP-tagged multi-spanning membrane protein 13g6 is found within the endoplasmic reticulum of Schizosaccharomyces pombe.

challenge
acceptableSCHPO:GRP782014-04-05 16:52:16 CDTGO:0005783 endoplasmic reticulum (C)PMID:8227200ECO:0000314 direct assay evidence used in manual assertion

Figure 6E: Confocal immunofluorescence microscopy results indicate that BiP protein is localized in the Endoplasmic Reticulum of S. pombe strain 972.

challenge
acceptableSCHPO:GRP782014-04-05 17:01:43 CDTGO:0005783 endoplasmic reticulum (C)PMID:10641037ECO:0000314 direct assay evidence used in manual assertion

Figure 3A: immunofluorescence microscopy results indicate that BiP1 is localized in the Endoplasmic reticulum.

challenge
acceptableSCHPO:GMA122014-04-05 18:03:29 CDTGO:0031228 intrinsic component of Golgi membrane (C)PMID:7522655ECO:0000314 direct assay evidence used in manual assertion

Figure 9 A-D: examining an immunoelectron microscopy results, it is inferred that alpha 1,2 Galactosyltransferase enzyme is a transmembrane protein located in Golgi apparatus in wild type fission yeast (Schizosaccharomyces pombe).

challenge
updatedbyinstructorSCHPO:KLP32014-04-05 20:21:53 CDTGO:0005881 cytoplasmic microtubule (C)PMID:10641037ECO:0000314 direct assay evidence used in manual assertion

Figure 7D: according to the Immunofluorescence microscopy results, HA-tagged Klp3 fusion protein in wildtype Schizosaccharomyces pombe is localized to the cytosolic microtubules.

challenge
acceptableSCHPO:YPT52014-04-06 13:37:48 CDTGO:0031901 early endosome membrane (C)PMID:8374169ECO:0000314 direct assay evidence used in manual assertion

figure 9: immunofluorescence microscopy results indicate that ypt5 protein in located in early endosome membrane of transfected HeLa cells.

This annotation is IDA because authors' intention is to prove that both proteins (reb5 and ypt5) are homologs and replacing them in HeLa cell line does not affect any phenotype.

challenge
acceptableSCHPO:YPT52014-04-06 13:57:38 CDTGO:0034058 endosomal vesicle fusion (P)PMID:8374169ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 7: Electron microscopy results indicates that ypt-5 gene disruption results in abnormal vesicle formation (which eventually will bind to endosome and cause abnormal endosome morphology) and disrupted membrane sturcture in mutant Schizosaccharomyces

pombe.

challenge
unacceptableSCHPO:YPT52014-04-06 14:16:15 CDTGO:0034058 endosomal vesicle fusion (P)PMID:8374169ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6: disruption in ypt5 gene (produces protein that regulates vesicle fusion to endosome) disrupts protein function and results in abnormal cell morphology.

challenge
unacceptableYEAST:AZR12014-04-06 15:46:41 CDTGO:0031226 intrinsic component of plasma membrane (C)PMID:11113970ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Azr1-GFP fusion protein is found in the plasma membrane in wild type baker's yeast.

challenge
unacceptableYEAST:HYM12014-04-06 17:48:01 CDTGO:0044732 mitotic spindle pole body (C)PMID:16325501ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 A-B: Pmo25 knockout mutant results in oval-shaped cells when compared to wild type strain with intact Pmo25 which result in elongated cell shape. This means protein Pmo25p is essential for polarized growth.

challenge
updatedbyinstructorYEAST:HYM12014-04-06 17:48:01 CDTGO:0044732 mitotic spindle pole body (C)PMID:16325501ECO:0000314 direct assay evidence used in manual assertion

Figure 2

challenge
unacceptableYEAST:HYM12014-04-06 17:53:15 CDTGO:0044732 mitotic spindle pole body (C)PMID:6325501ECO:0000314 direct assay evidence used in manual assertion

Fig. 2A-D: time lapse pictures show that GFP tagged Pmo25p (in wild type strain)is a component of single spindle pole body found during cytokinesis.

challenge
requireschangesYEAST:MOB22014-04-06 17:58:10 CDTGO:0005938 cell cortex (C)PMID:16325501ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 4C: Mob2p localizes to cell cortex.

challenge

acceptable:10
unacceptable:8
requires_changes:1
flagged:0

Annotations challenged by Rdesai525

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
unacceptableGrolfe,
Team Pancakes At Nite
HUMAN:CIB1GO:0051592 - response to calcium ion (P)PMID:20473878ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
unacceptableKdiawara,
Team Lobster Soul
HUMAN:CH60GO:0046789 - host cell surface receptor binding (F)PMID:12496435ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4
updatedbyinstructorChong,
Team Kitten Me Right Meow
HUMAN:SC6A4GO:0051610 - serotonin uptake (P)PMID:7681602ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorElizabethASchultz,
Team Designer Genes
EBVB9:LF2GO:0010629 - negative regulation of gene expression (P)PMID:17446270ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Rdesai525
unacceptableSarah.budde,
Team A-Squared
HELPX:Q9JP55GO:0016337 - single organismal cell-cell adhesion (P)PMID:8516329ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorHardin57,
MichSt14A 9
VIBAL:D0WWP7GO:1902573 - positive regulation of serine-type peptidase activity (P)PMID:22130678ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
unacceptableWiestcar,
MichSt14A 53
ARATH:B3GT1GO:0080022 - primary root development (P)PMID:23527103ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
unacceptableWiestcar,
MichSt14A 53
ARATH:B3GT1GO:0015995 - chlorophyll biosynthetic process (P)PMID:23527103ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
updatedbyinstructorGrevever,
MichSt14A 8
TANCI:H6U1I8GO:0016412 - serine O-acyltransferase activity (F)PMID:22385412ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorCermakme,
MichSt14A 5
CAMJE:Q0PBH4GO:1902210 - positive regulation of bacterial-type flagellum assembly (P)PMID:12055288ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
acceptableMcfadd48,
MichSt14A 40
BACSU:MCPCGO:0050918 - positive chemotaxis (P)PMID:23038252ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorPawlos25,
MichSt14A 42
SYNP2:RBSGO:0016984 - ribulose-bisphosphate carboxylase activity (F)PMID:6408075ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3

fixed by Rdesai525
updatedbyinstructorZielin64,
MichSt14A 54
PLAF4:KC1GO:0004674 - protein serine/threonine kinase activity (F)PMID:24658579ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

fixed by Rdesai525
unacceptableRdesai525,
Team Texas Aggies
YEAST:HYM1GO:0044732 - mitotic spindle pole body (C)PMID:16325501ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorRdesai525,
Team Texas Aggies
YEAST:HYM1GO:0044732 - mitotic spindle pole body (C)PMID:16325501ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

fixed by Rdesai525
unacceptableLzeihen,
delta3
CORGL:PKN1GO:0004674 - protein serine/threonine kinase activity (F)PMID:18442973ECO:0000247 sequence alignment evidence used in manual assertionchallengeC: 1
requireschangesLzeihen,
delta3
CORGL:Q6M3Q8GO:0004674 - protein serine/threonine kinase activity (Figure 3 in text)PMID:18442973IDA: Inferred from Direct Assay challengeC: 1
requireschangesLzeihen,
delta3
CORGL:Q6M299GO:0004674 - protein serine/threonine kinase activity (Figure 3 in text)PMID:18442973IEP: Inferred from Expression Pattern challengeC: 1
requireschangesRdesai525,
Team Texas Aggies
YEAST:MOB2GO:0005938 - cell cortex (C)PMID:16325501ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
unacceptableCford,
Team What the Fox Says
HUMAN:ENW1GO:0001948 - glycoprotein binding (F)PMID:15452578ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

4 annotations fixed by Rdesai525