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GO:0016235aggresomePMID:21223600IMP: Inferred from Mutant Phenotype C
This annotation made on page: EBOZM:NCAP
By: Oherrera (group Team That Will Beat You!!!) on 2011-10-23 11:56:54 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeOherrera,
Team That Will Beat You!!!
2011-10-25 19:21:14 CDT

I believe the annotation is still valid because of the paragraph in the paper:

"Ebola NP expressed in isolation accumulated in large cytoplasmic aggregates (Figure 3B). Staining for the intermediate filament protein, vimentin revealed these NP aggregates were lined with vimentin. Aggresomes are perinuclear structures lined with vimentin, which recruit molecular chaperones and proteosomes. They are believed to regulate protein folding and degradation of misfolded proteins [32]. Therefore, the NP association with vimentin here suggests the NP was present in aggresome-like structures."

While I may have included this in the notes, the rules of the competition do not require extended notes, just the figure or table. Therefore this annotation is still valid.

0
ChallengeHessa,
Team Biology Bandits
2011-10-23 20:22:03 CDT

Figure 3 B:

" HEK293T cells were transfected with 10 μg Ebola NP plasmid for 24 hours. Cells were fixed and stained for DNA using Hoechst (blue), NP using a mouse monoclonal antibody targeting NP (red) and vimentin using a mouse monoclonal antibody targeting vimentin (green). Scale bar represents 5 μm. Upper and lower side panels show the vimentin and DNA fluorescence and the NP and DNA fluorescence respectively, from the boxed region in the image."

This should be explained more, how was the Go term: aggresome derived from this evidence?

0
ChallengeHessa,
Team Biology Bandits
2011-10-23 20:11:34 CDT

I think more information should be added to the notes section with regards to the experimental evidence.

1
Private
Assessment
Bmcintosh2011-11-22 10:50:46 CSTYou need to be an instructor to view these notes.Unacceptable
Public
Assessment
Bmcintosh2011-11-22 10:49:29 CST

There is a larger problem with this annotation.

Unacceptable