GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Cacao

Jump to: navigation, search
GO:0008933lytic transglycosylase activityPMID:24690638IDA: Inferred from Direct Assay F
This annotation made on page: 9CAUD:I1TEH9
By: Eric (group RedSU18) on 2018-07-14 09:16:50 CDT.



TeamPoints
RedSU185




You must be logged in to challenge this annotation.

Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Ivanerill2018-07-24 12:40:29 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-07-18 12:44:31 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0008932 lytic endotransglycosylase activity PMID:24690638 IDA: Inferred from Direct Assay F Fig 4. Author analyzes endolysin target sites by isolating peptidoglycan from E. coli and treating it with SPN9CC endolysin. Different byproducts of the reactions were analyzed to determine glycosidase, amidase, and peptidase activity. Results conclusively showed that the lysin possesses glycosidase activity. Since glycosidase activity involves alteration of glycosides, it can be categorized as transglycosylase activity. The reaction product used to measure glycosidase activity was 1,6-anhydromuramic acid, which is indicative of cleavage within the peptidoglycan chain instead of cleavage at the ends. This shows that the lysin possesses endotransglycosylase activity. complete
CACAO 13298
on 9CAUD:I1TEH9
Flagged
Public
Assessment
Ivanerill2018-07-17 05:27:20 CDT

Check out if a more specific GO term might apply. Make your notes a bit more explicit: "the endolysin possesses glycosidase (and therefore transglycosylase) activity." Hence, how?

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original