User:Eric

CACAO Phage Hunters S2018

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	9CAUD:I1TEH9	2018-07-14 09:16:50 CDT	GO:0008932 lytic endotransglycosylase activity (F)	PMID:24690638	ECO:0000314 direct assay evidence used in manual assertion	Fig 4. Author analyzes endolysin target sites by isolating peptidoglycan from E. coli and treating it with SPN9CC endolysin. Different byproducts of the reactions were analyzed to determine glycosidase, amidase, and peptidase activity. Results conclusively showed that the lysin possesses glycosidase activity. Since glycosidase activity involves alteration of glycosides, it can be categorized as transglycosylase activity. The reaction product used to measure glycosidase activity was 1,6-anhydromuramic acid, which is indicative of cleavage within the peptidoglycan chain instead of cleavage at the ends. This shows that the lysin possesses endotransglycosylase activity.	challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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