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Cacao

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Contributes toGO:0016042lipid catabolic processPMID:18451045IDA: Inferred from Direct Assay P
This annotation made on page: 9VIRU:Q9FZR9
By: HPaudel (group Team Green B 2018) on 2018-04-05 13:10:55 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment		Ivanerill2018-05-02 09:19:42 CDTNo notes given.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-04-30 09:20:59 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0016298 lipase activity PMID:18451045 IDA: Inferred from Direct Assay F The protein Q9FZR9 is involved in lipolytic activity as shown by figure 3. It was hypothesized to have that activity based on this motif. Gly-Tyr-Ser-Gln-Gly. The enzymatic activity done invitro matches this hypothesis. The paper is investigating additional proteins to help with cell lysis using the specific bacteriophage MS6 a phage that infects Mycobacterium smegmatis. They specifically look at the product of gene LysB which is the protein in question. Figure 3 demonstrates the lipolytic activity by using the product of LysB to produce a zone of clearance in plates coated with triglycerides such as triolein. complete
CACAO 13198
on 9VIRU:Q9FZR9
Flagged
Public
Assessment		Ivanerill2018-04-30 06:12:20 CDT

The “contributes to” qualifi er is used to capture the molecular function of complexes when the activity is distributed over several subunits. I don't see evidence of this here.

Requires Changes
✔ Protein
✔ Publication
✗ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-04-15 19:00:28 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
Contributes to GO:0016298 lipase activity PMID:18451045 IDA: Inferred from Direct Assay F The protein Q9FZR9 is involved in lipolytic activity as shown by figure 3. It was hypothesized to have that activity based on this motif. Gly-Tyr-Ser-Gln-Gly. The enzymatic activity done invitro matches this hypothesis. The paper is investigating additional proteins to help with cell lysis using the specific bacteriophage MS6 a phage that infects Mycobacterium smegmatis. They specifically look at the product of gene LysB which is the protein in question. Figure 3 demonstrates the lipolytic activity by using the product of LysB to produce a zone of clearance in plates coated with triglycerides such as triolein. complete
CACAO 13198
on 9VIRU:Q9FZR9
Flagged
Public
Assessment		Ivanerill2018-04-14 20:18:13 CDT

Describe the experiment with more detail. GO:0016042 is biological process. This is in vitro, demonstrating activity. Find a GO term that describes this. hint: a lipolytic enzyme is a lipase.

Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original


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